Sequences from confirmation amplicon


Confirmation sequences for line SALK_047462 and hit At4g33910 are shown.
Line ID SALK_047462
Locus At4g33910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16258184
Primers used for PCR 12rr - LBb3, show primer details
   
Sequence Name 46-K116392-SALK_047462C-12rr
GenBank Accession LN485419 [GenBank]
Sequencing primer gene specific primer, 12rr: 5'-CTGGTCAACGAGATCTGATCATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K116392-SALK_047462C-12rr
ACGAAAGGCGTCCCGATATTGAGGTTCGTTACGCCGGCGAAGATTTTCATCGGAAACTTA
TCTGATCGTTTACGCCGGCGAAGATTTCCATCGGAAACTTAATCTGATCGGAAGGAAGAA
GGCTCGATGAAGAGCAGATTGAAGAGCTACAGGAGGAAGAAGCTAGGGTTAGCCACAGTT
ATCGTCTTCTGTTCTCTCTGTTTCCTCTTCGGTTTCTATGGCTCCACTCTACTTTCTCAG
GTGTGGAAACTCTCTGATCTTTTACTCTGTACTCTTTAGATGTGATTGGAGTCTTTAGCT
TGGTTTTGTGACTAATCGAGGGTTCTATGTTTGGGATCAGAATGTGCCTCGTGTTAAACC
CAGGTTGAGAATGTTGGATATGGTTGAGAATGGGGAAGAAGAAGCTAGCTCGATGCCTCA
TGGTGTTACTGGAGAAGAATCTATTGGATCGATTCCTTTTCAGGTCTGATTTTGTTGCTA
ATTTTTTGACTTTTAAGATGTTTGGATTGGCAAATTAGGTGAAATCGATTAGTTAGGGCA
AGATTGTGCATTTGATTGTCAATTTTAAACCAGGATATATTGTGGTGTAAACAAATTGAC
GCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCA
GCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
   
Sequence Name 21-K116391-SALK_047462C-R210
GenBank Accession LN484889 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116391-SALK_047462C-R210
AATATATCCTGGGTTAAAATTGACAATCAAATGCACAATCTTGCCCTAACTAATCGATTT
CACCTAATTTGCCAATCCAAACATCTTAAAAGTCAAAAAATTAGCAACAAAATCAGACCT
GAAAAGGAATCGATCCAATAGATTCTTCTCCAGTAACACCATGAGGCATCGAGCTAGCTT
CTTCTTCCCCATTCTCAACCATATCCAACATTCTCAACCTGGGTTTAACACGAGGCACAT
TCTGATCCCAAACATAGAACCCTCGATTAGTCACAAAACCAAGCTAAAGACTCCAATCAC
ATCTAAAGAGTACAGAGTAAAAGATCAGAGAGTTTCCACACCTGAGAAAGTAGAGTGGAG
CCATAGAAACCGAAGAGGAAACAGAGAGAACAGAAGACGATAACTGTGGCTAACCCTAGC
TTCTTCCTCCTGTAGCTCTTCAATCTGCTCTTCATCGAGCCTTCTTCCTTCCGATCAGAT
TAAGTTTCCGATGGAAATCTTCGCCGGCGTAAACGATCAGATAAGTTTCCGATGAAAATC
TTCGCCGGCGTAACGAACCTCAATATCGGGACGCCTTTCGTCGATCCAATGAAGCACCAC
AAATTTCTATTTACTTTAAAATATATTGTTTTATAGTTTAATGATCAG

Last Updated on 06.12.2018 16:11