Sequences from confirmation amplicon


Confirmation sequences for line SALK_047557c and hit At1g29820 are shown.
Line ID SALK_047557c
Locus At1g29820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10438426
Primers used for PCR 35cv - LBb3, show primer details
   
Sequence Name 87-K109033-SALK_047557C-35cv
GenBank Accession LN486304 [GenBank]
Sequencing primer gene specific primer, 35cv: 5'-TGAGATTTTGGTTTCTCGAAAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K109033-SALK_047557C-35cv
ATAGAGATCACATTTAAGTTGAATTCTCTAGAAACCAGTAGAAACTTTGTATGATCTTAT
CAGTCGAATTTGTGTATCTCCTTATCTCAATAAACTTGTTCTTTCAATCATGTTCATCTT
TGGATTATAATTACTTTCTGATGAACTTGCTATTTCTAATTATAAATTCTGGGCCTACTC
TAAGGTTACTTTGTTTGAAACCTCATCTTCTTGAGTCTAAAGTTTTGGAATTTATGCTAT
TCCGTACTTTAATTATGTTACTTGCATGTTATTTTCTTGTATTTGTTGCGTCAAATTTGC
AGTTTTGGTGTTGTTGTTAGTTTGCTGCTTTGATCTCATACAATGAGCTTTGTTATGTTT
GAATTCTCTACAGAGTTGTGTTAAATCAACATCTTTCTGTTTGATCTTGCAGAGTTTAAA
TTATCTGTCTTGAGAAGCTGGTAGATTTTTCTATTGATGAACAATGGGTGAGAACGGGGA
GAATCATTCAGTCTCTCAAGAGAGTAAATCCAAGAAGCTTGAGAGTAAAAATCATTCAGG
TGTTCCCAAGAGCGATCTTTGGACTGATGGGATTATTTGTGCCTTTGAGTTT
   
Sequence Name 71-K109032-SALK_047557C-R210
GenBank Accession LN486024 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K109032-SALK_047557C-R210
GACTGATTGGTCATCTTCTTGCTTCAAGCACCTTTCTCAAGTTGTTTTGACTCTACATGT
TTTTTCGGACCTCGAATAAACTCAAAGGCACAAATAATCCCATCAGTCCAAAGATCGCTC
TTGGGAACACCTGAATGATTTTTACTCTCAAGCTTCTTGGATTTACTCTCTTGAGAGACT
GAATGATTCTCCCCGTTCTCACCCATTGTTCATCAATAGAAAAATCTACCAGCTTCTCAA
GACAGATAATTTAAACTCTGCAAGATCAAACAGAAAGATGTTGATTTAACACAACTCTGT
AGAGAATTCAAACATAACAAAGCTCATTGTATGAGATCAAAGCAGCAAACTAACAACAAC
ACCAAAACTGCAAATTTGACGCAACAAATACAAGAAAATAACATGCAAGTAACATAATTA
AAGTACGGAATAGCATAAATTCCAAAACTTTAGACTCAAGAAGATGAGGTTTCAAACAAA
GTAACCTTAGAGTAGGCCCAGAATTTATAATTAGAAATAGCAAGTTCATCAGAAAGTAAT
TATAATCCAAAGATGAACATGATTGAAAGAACAAGTTTATTGAGATAAGGAGATACACAA
ATTCGACTGATAAGATCATACAAAGTTTCTACTGGTTTCTAGAGAATTCAACTTAAATGT
GATCTCTAATTTAGTCAAAAATCTCCAATAAAGAAACGTAAAACTTTCGAGAAACCAAAA
TCTCAA

Last Updated on 06.12.2018 16:11