Sequences from confirmation amplicon


Confirmation sequences for line SALK_047689c and hit At4g25980 are shown.
Line ID SALK_047689c
Locus At4g25980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13190720
Primers used for PCR 06tw - LBb3, show primer details
   
Sequence Name 16-K116922-SALK_047689C-06tw
GenBank Accession LN484726 [GenBank]
Sequencing primer gene specific primer, 06tw: 5'-CAGGTAAGAAATCATAGAGCCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116922-SALK_047689C-06tw
AGCTGATACGGAAAGTAGACCACTCAGGTTTAGATCTCTTAAATTCTTAAAACTAGTGAC
AATTTTTTTTGTCAAAGTTTTCTCATATTGAAACTTGAAATGTAAACTAAATAAAAAAAT
GACGCAAACTGAGCCGGACCAGGTTCAAAATGCATGATCACTACTGGCTTAGACTCAGCA
TGTTGATGGTTTCGTCTTTGTTTAGTCACACGTGAAAGTTGATAATTTCACGATCGTATC
GAGATCAGTATTGGTTTTTGATGAAAATAAAAATAAAAAAAAATCAATACTCTATAGAAT
GGGGCCGTTAGATATTTATGACGTTTTATCAGATTAATTGCCGATCGTGTAACAGGCCCA
TTTTCGGGCAAATTGACGGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 05-K116921-SALK_047689C-R210
GenBank Accession LN484408 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116921-SALK_047689C-R210
GTCGTCATTTGCCCGAAATGGGCCTGTTACACGATCGGCAATTAATCTGATAAAACGTCA
TAAATATCTAACGGCCCCATTCTATAGAGTATTGATTTTTTTTTATTTTTATTTTCATCA
AAAACCAATACTGATCTCGATACGATCGTGAAATTATCAACTTTCACGTGTGACTAAACA
AAGACGAAACCATCAACATGCTGAGTCTAAGCCAGTAGTGATCATGCATTTTGAACCTGG
TCCGGCTCAGTTTGCGTCATTTTTTTATTTAGTTTACATTTCAAGTTTCAATATGAGAAA
ACTTTGACAAAAAAAATTGTCACTAGTTTTAAGAATTTAAGAGATCTAAACCTGAGTGGT
CTACTTTCCGTATCAGCTGCTCACACTATCGGAACAACGGCCTGCTTCTTCATGTCCAAA
CGGCTCTATGATTTCTTACCTG

Last Updated on 06.12.2018 16:11