Sequences from confirmation amplicon


Confirmation sequences for line SALK_048033 and hit At4g13460 are shown.
Line ID SALK_048033
Locus At4g13460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7826256
Primers used for PCR 09ef - LBb3, show primer details
   
Sequence Name 20-K111273-SALK_048033C-09ef
GenBank Accession LN484846 [GenBank]
Sequencing primer gene specific primer, 09ef: 5'-TGGATTTAGCTTGAAAGGTGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K111273-SALK_048033C-09ef
TAACTCGTCAACCCATTTCGCTTCTCGTTGCAAAAAATGGGTTCTTCTCACATTCCTCTT
GATCCGTCTCTAAATCCGTCACCTTCACTGATCCCAAAGCTTGAACCAGTCACTGAATCA
ACCCAAAACTTGGCCTTTCAACTTCCAAACACAAACCCACAAGCCCTAATTTCATCAGCC
GTCTCCGATTTCAACGAAGCCACAGACTTTTCCTCAGATTACAACACCGTCGCCGAGTCA
GCCCGGTCTGCTTTCGCTCAACGGCTTCAACGTCACGATGATGTTGCGGTTCTTGATTCC
TTAACCGGAGCAATCGTACCGGTTGAGGAGAATCCGGAACCGGAACCGAATCCTTACTCA
ACCAGTGACTCTTCACCGTCGGTTGCGGACAACTGATTGTGGTGTAAACAAATTGACGCT
TAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 04-K111272-SALK_048033C-R210
GenBank Accession LN484364 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K111272-SALK_048033C-R210
CATTTGTTACCCACAATCAGTTGTCCGCAACCGACGGTGAAGAGTCACTGGTTGAGTAAG
GATTCGGTTCCGGTTCCGGATTCTCCTCAACCGGTACGATTGCTCCGGTTAAGGAATCAA
GAACCGCAACATCATCGTGACGTTGAAGCCGTTGAGCGAAAGCAGACCGGGCTGACTCGG
CGACGGTGTTGTAATCTGAGGAAAAGTCTGTGGCTTCGTTGAAATCGGAGACGGCTGATG
AAATTAGGGCTTGTGGGTTTGTGTTTGGAAGTTGAAAGGCCAAGTTTTGGGTTGATTCAG
TGACTGGTTCAAGCTTTGGGATCAGTGAAGGTGACGGATTTAGAGACGGATCAAGAGGAA
TGTGAGAAGAACCCATTTTTTGCAACGAGAAGCGAAATGGGTTTGACGAGTTTATCAGAG
AAATCGAAGAAATGGGTTTTGTTTGGAGAACACCTTTCAAGNTAAATCC

Last Updated on 06.12.2018 16:11