Sequences from confirmation amplicon


Confirmation sequences for line SALK_048245c and hit At5g53750 are shown.
Line ID SALK_048245c
Locus At5g53750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21817389
Primers used for PCR 23ea - LBb3, show primer details
   
Sequence Name 28-K110755-SALK_048245C-23ea
GenBank Accession LN485082 [GenBank]
Sequencing primer gene specific primer, 23ea: 5'-AATCAGATTCTGTGCTCCTTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K110755-SALK_048245C-23ea
AAAACGGACTTTAGGAAACGAACAAAAAACAGAGCATTAAGAAACAGAACCAATGTCTGG
ATTTGTAACATACCTGCAAGAAGATTGAACATGGACGACGAGGGCACGAGATTTGGGAAG
AAGAACAGAGACAGATGCGTCGAAAGCAGAGGAAAGAGACAAAACATTGTTGTCGAATTT
GGATAGGTAACAGATTACATCAGCCATACAGATCTTACCCAAACACTCACACTTATCATT
ATCATCTGTTTTCTCATCGTGATTACAGCTCCATACGGTGAGGAACGGTTCGTCAGAGGA
TTTGAGAGCGGCGATGGCGTCAGCTACGGTGGCTGTGGCGACGGAGAGACACCGTAAAGG
TGGCTTACCGATACAGAGGTCAGATAATTCGTGAGACAAAAGGGTTAATGCCATAAAAGA
AGAATTTTTTTTTTTTTTTTTCCAAAAATGGTGAGGGAAAAATGGGAAAAAAAAACCGGG
GAAAAATGGGTTTTTAAGGGAAAAAAAAAAGGGGCCCGGGTTTAAAGTCCTTAACCCCAA
ACTTTTTTTTGGCCCTTAAAACACTTTAAAACCCCTTGGGGAACTTTTTTAAGGACGGGG
GGGGGTTTTTTTTTTCCCCGGGGAAAGGGGAAACGGTTGTTTCCCCTTCCCCCCCGGGCC
CTAAAAAATTTCCACAAGGGGCCCCACCTGGGTTGCCCCCAGCGGGAAAAAACCCGGTTG
GAGGGGGGTCCCAA
   
Sequence Name 20-K110754-SALK_048245C-R210
GenBank Accession LN484845 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K110754-SALK_048245C-R210
GTAGTTTTGTGTTTTGTACTTTAACACCGTACCCATCTCTCTTCTCCTTTATAACCACTT
CTTCCTCTGTCTTCTCTCTCCATCTTCTCCTTCACATTCTCTGCAAAAAAAAAAAAAAAA
TTTTTTTTTTTGGGGATTAACCCTTTTGGTCCCAAAAATTTTCGGCCCTTGGTTGGGGAA
ACCCCCTTTTGGGGGGTTTCCCCGCCCCCCCGCCCCCGGAAGTGAACCCCTCCCCCCTTT
TAAAACCCTTGGAAAAACGGTTCCCCCCGGTGGGGGGTGGTATCCCCAAGAAAAAAACAA
ATAAAATGGAAAGGGGGGGGGGTTGGGGAAAAATTGGTGGGGGGGAGGAATTTGGTTCCC
TTCCAAATTCCAAAAAAAGGGTTTGGTCTTTTCCCCGGGTTTTGAACGCACTTGTTTTGG
TTTTTTTCCCAAAATCTGGGGCCCCGGCGGCCAGGGTTAAATTTTTTGGGGGGTTTTTAA
AAAACCCAAACTTGGGTCGGGTTTCAAAGGGCCCGGTTTTTTGGTCCGGTTCCCAAAACC
CCGGTTTTGGCGTTTGGATGGAACCTTTGGTTCGAAAATCAAAGGGGCCCCAAATTTTAT
TA

Last Updated on 06.12.2018 16:11