Sequences from confirmation amplicon


Confirmation sequences for line SALK_048982c and hit At3g05200 are shown.
Line ID SALK_048982c
Locus At3g05200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1477303
Primers used for PCR 24kw - LBb3, show primer details
   
Sequence Name 20-K115143-SALK_048982C-24kw
GenBank Accession LN484857 [GenBank]
Sequencing primer gene specific primer, 24kw: 5'-GTAATCTCCTCGAATCGACTTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115143-SALK_048982C-24kw
ACCACCGGTTCGGATTCACCACAACCTGCTGCAATTCAAGATCCGGTTCAGTTCCTCCCG
GGTTCACTGACTCACCTTCAGCTACCTGTTCAGCAAGATTCGCTCTGCAAACGGGACAAG
TCACGTGAGCCTCGAGCCAAGCATCGATGCAGTGCGGATGAAACACGTGATCACACTTGG
GAAGCAAACGCAGCGTTTCGTCGTCTTCGAACTCGTTCAAACATATCGCACACTCTAACT
CTCCTTTACCAAGCTTCTGCGTTTTCACATCTGAGTACAAGAACGTAGGAAAAGTCTCGA
CAACTGAAACGTCGAGACCACGCGCCGCCGCGTTGACGGTTGCTCTGGATCTAGCTCCAC
CAGCTGGAGAAACACCGGCGTCTGGTACGCCTGAGCAGTGACGGAAGTAGATAGAGAAGA
AGCCCATGAAGAAGAGAGCAGCGATTAGGATCACGACGATCACTGCCATGGCTGGACTTA
GTCTGCCGTAGTTATACGGTTGATTTGTTGGACCGGGCTGTGATTGACTCGCTGCTAGAT
CCGCTGATGATAATATAAGCAAGAAAACAATCGGCAAAACGCCGGCNAACGCCATATGAT
CGGAGCTTCTCATGATTTTGAGTTTTTTTTTGTGTGTTTATTTGGCTCTGGGAGGAGAAG
AAAGTGAGTTTATAGCGTAAAAAAAGAATAACACATTATATATTGTGGTGTAAACAAATT
GACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAGAGAGTT
GCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCGGTTTGATGGTGGTT
   
Sequence Name 04-K115142-SALK_048982C-R210
GenBank Accession LN484378 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115142-SALK_048982C-R210
TTTGTTTACCCCAATATATAATGTGTTATTCTTTTTTTACGCTATAAACTCACTTTCTTC
TCCTCCAAGAGCCAAATAAACACACAAAAAAAAACTCAAAATCATGAGAAGCTCCGATCA
TATGGCGTTTGCCGGCGTTTTGCCGATTGTTTTCTTGCTTATATTATCATCAGCGGATCT
AGCAGCGAGTCAATCACAGCCCGGTCCAACAAATCAACCGTATAACTACGGCAGACTAAG
TCCAGCCATGGCAGTGATCGTCGTGATCCTAATCGCTGCTCTCTTCTTCATGGGCTTCTT
CTCTATCTACTTCCGTCACTGCTCAGGCGTACCAGACGCCGGTGTTTCTCCAGCTGGTGG
AGCTAGATCCAGAGCAACCGTCAACGCGGCGGCGCGTGGTCTCGACGTTTCAGTTGTCGA
GACTTTTCCTACGTTCTTGTACTCAGATGTGAAAACGCAAAAGCTTGGTAAAGGAGAGTT
AGAGTGTGCGATATGTTTGAACGAGTTCGAAGACAACAAAACGCTGCGTTTGCTTCCCAA
GTGTGATCACGTGTTTCATCCGCACTGCATCGATGCTTGGCTCGAGGCTCAAGTGACTTG
TCCCGTTTGCAGAGCGAATCTAGCTGAACAGGTNGCTAAAGGTGAGTCAGTTGAACCGGG
AGGAACTGAACCGGATCTTGAATTGCAGCAGGTTGTGGTGAATCCTGAACCGGTGGTTAC
TGCACCGGTACCGGAACAATTGGTGACTAGTGAAGTCGATTC

Last Updated on 06.12.2018 16:11