Sequences from confirmation amplicon


Confirmation sequences for line SALK_049248c and hit At1g75990 are shown.
Line ID SALK_049248c
Locus At1g75990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28524769
Primers used for PCR 48cy - LBb3, show primer details
   
Sequence Name 44-K109566-SALK_049248C-48cy
GenBank Accession LN485369 [GenBank]
Sequencing primer gene specific primer, 48cy: 5'-GTGAATCTGAGAACCTGTGATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K109566-SALK_049248C-48cy
TGAGGAGGAGAATCGGAAGTTCTCCATATCTGAAAGCAGAAGCTTTCTCAATTCGTCTTC
CTCTTCATCTTCTTGTGTAGCTAGCTGCTGCTTCTGTTGGTCGTCGCAATCTTCTACTTC
GTCGTTTCTCTTTGGGTTAGTTAATTCGATTGTATCCTCAATTTCTGGAAACTTCGCTAC
ATCAAGCTTCAGAATCTCCATTGTTTCGACTGTTTAACCTTTGACGGCGAAAAAACAATC
GGAGTTACTTCGCCGATTTGTAATTACATAACTAATGGGCCTAAAGGCCCATCTACTGAT
CTTGGGTTTGAATCATGTTCATGTTCTTCTATATTACTAACTTCACTTCTTCAGTGAATT
TATTTACCAGAATTTTGATCCCAATGTGGACTTGTGATAAAGTTTGAGATTTTGTTTTTT
CATAAAGTAAAAGAACTGATGTGCATAATAACAAGAAAAAGTCAGATAATTCAAAATAAC
AAAAGTGTTAACAGACAAAAACAGTAGAATTTGAAACAAATCTAGAAATCATCATCGTCT
TCCTCAGCCATGTATTTAGCGAGCTCCTCTTCTTGCTGTTTCATCTCCCTTCTTTTCTCC
TCGCTCTCTTTCTCCCTGTGCGTGTTTGGCGGGAACCTCAGTGCTCTAACCGCTTCATTG
TGCATATTGTGGATATATTGTGGTGTAAACAAATTGACGCTNAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAG
CTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG
CCCCAGCAGGCGAAAATCCTGTTTGATGG
   
Sequence Name 35-K109565-SALK_049248C-R210
GenBank Accession LN485217 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K109565-SALK_049248C-R210
CGTCATTTGTTTACCCACAATATATCCACAATATGCACAATGAAGCGGTTAGAGCACTGA
GGTTCCCGCCAAACACGCACAGGGAGAAAGAGAGCGAGGAGAAAAGAAGGGAGATGAAAC
AGCAAGAAGAGGAGCTCGCTAAATACATGGCTGAGGAAGACGATGATGATTTCTAGATTT
GTTTCAAATTCTACTGTTTTTGTCTGTTAACACTTTTGTTATTTTGAATTATCTGACTTT
TTCTTGTTATTATGCACATCAGTTCTTTTACTTTATGAAAAAACAAAATCTCAAACTTTA
TCACAAGTCCACATTGGGATCAAAATTCTGGTAAATAAATTCACTGAAGAAGTGAAGTTA
GTAATATAGAAGAACATGAACATGATTCAAACCCAAGATCAGTAGATGGGCCTTTAGGCC
CATTAGTTATGTAATTACAAATCGGCGAAGTAACTCCGATTGTTTTTTCGCCGTCAAAGG
TTAAACAGTCGAAACAATGGAGATTCTGAAGCTTGATGTAGCGAAGTTTCCAGAAATTGA
GGATACAATCGAATTAACTAACCCAAAGAGAAACGACGAAGTAGAAGATTGCGACGACCA
ACAGAAGCAGCAGCTAGCTACACAAGAAGATGAAGAGGAAGACGAATTGAGAAAGCTTCT
GCTTTCAGATATTGGAGAACTTCCGATTTCTCCTCCTTCAGCTACACAAGTTCATTTTGT
TTCTTACTTCATCACAG

Last Updated on 06.12.2018 16:11