Sequences from confirmation amplicon


Confirmation sequences for line SALK_049540c and hit At3g50410 are shown.
Line ID SALK_049540c
Locus At3g50410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18710109
Primers used for PCR 81sm - LBb3, show primer details
   
Sequence Name 43-K116586-SALK_049540C-81sm
GenBank Accession LN485358 [GenBank]
Sequencing primer gene specific primer, 81sm: 5'-CTTCATAGGGATGAAAAACAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K116586-SALK_049540C-81sm
GCTAAACAATAATAAACAAATCCTCATAAAAAGACAAATTTCAAAGACAAGAGAGAAGAG
AGAGATAGAGAGCGTGGCCTCTAATCAACCCAACACCCTCTGTCAAAAACAAGCCTGACT
TTCTTCTCCATCCCCATTTCTATCTGCGCATGTCTCTCTCTTTCTTCTCCATAAAAAACC
AAATTCTCACTCTCTCATACCCTAAAGTTTCAACCTTTAAACCCTAAAACCAAAAAAGCT
TCAGTCTTTTGCATCACTTCCCTTAAAGCTTCAACCTTTCTCCGATTATGCCGACGTCTG
ACTCCGGTGAACCACGGCGAATAGCTATGAAACCTAACGGCGTAACAGTTCCGATTTCTG
ACCAGCAAGAACAGCTTCCATGTCCTCGTTGTGACTCATCCAACACTAAGTTCTGTTACT
ACAACAACTACAACTTCTCTCAGCCTCGTCACTTCTGCAAAGCTTGTCGTCGTTACTGGA
CTCACGGTGGTACTCTCCGTGACGTTCCTGTCGGTGGTGGTACTCGTAAAAGCGCGAAAC
GTTCCCGCACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
   
Sequence Name 26-K116585-SALK_049540C-R210
GenBank Accession LN485040 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116585-SALK_049540C-R210
GCTTTCAGAGTACCACCACCGACAGGAACGTTCACGGAGAGTACCACCGTGAGTCCAGTA
ACGACGACAAGCTTTGCAGAAGTGACGAGGCTGAGAGAAGTTGTAGTTGTTGTAGTAACA
GAACTTAGTGTTGGATGAGTCACAACGAGGACATGGAAGCTGTTCTTGCTGGTCAGAAAT
CGGAACTGTTACGCCGTTAGGTTTCATAGCTATTCGCCGTGGTTCACCGGAGTCAGACGT
CGGCATAATCGGAGAAAGGTTGAAGCTTTAAGGGAAGTGATGCAAAAGACTGAAGCTTTT
TTGGTTTTAGGGTTTAAAGGTTGAAACTTTAGGGTATGAGAGAGTGAGAATTTGGTTTTT
TATGGAGAAGAAAGAGAGAGACATGCGCAGATAGAAATGGGGATGGAGAAGAAAGTCAGG
CTTGTTTTTGACAGAGGGTGTTGGGTTGATTAGAGGCCACGCTCTCTATCTCTCTCTTCT
CTCTTGTCTTTGAAATTTGTCTTTTTATGAGGATTTGTTTATTATTGTTTAGCTTTGGAA
TTGTTGAGTCGGCTATGGGATTATTGTAAACAGTGAAATGCTCTGTTTT

Last Updated on 06.12.2018 16:11