Sequences from confirmation amplicon


Confirmation sequences for line SALK_049590c and hit At5g22400 are shown.
Line ID SALK_049590c
Locus At5g22400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7425315
Primers used for PCR 73hw - LBb3, show primer details
   
Sequence Name 14-K113018-SALK_049590C-73hw
GenBank Accession LN484670 [GenBank]
Sequencing primer gene specific primer, 73hw: 5'-AGGTTCTACAACTCGAAACAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K113018-SALK_049590C-73hw
CCACCCCAAAATTGCAGGACAAGATCATTGTTGTGAGGAGACTTTTAAGAGCAATTCAAT
AAAAAGGTAGGATCTTTGGACAATGTACCTGGCGCTTGGAGCTCTTCTAGGAACTTCAGG
CTCGAATTCAACAGGCAAACCCAAGAAGCCATTGAAACGATCAAAGGTAACGTGCGCCAC
GTGTCTGACATTGGTAGGCCATCCAATCTCCATGCTACAAAGCTCCCTCCGGTTACTCTT
GCAAGAAATCAAAGATCTCCTGAAAATGGCAACAAGAAGAGCCAACAACGAAATCTGATC
TTCCCCACCATCGTCTTCTTCTCCTCCATCGGTACTGCTCCGTCTTCTCAAATCTTGTTC
TTCGATTGAGGCATGAAAGTCAACATCTTGATCGAATCTAGCAACTGGGTTTCTCCGGTT
GTGGTCAGAGCTAATCAAGAGAGTCGCATTAGAGCGAGAGGCGTAAGATAAAGAAGAAGG
TGAAGGAGAAGAAGAAGAAGAAGAAGATGAATGAGAAGCGCTTGGAGATGAAGGATATAT
TGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATG
TACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGC
CTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATC
CTGTTTGATGGTGGTTCCG
   
Sequence Name 06-K113017-SALK_049590C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K113017-SALK_049590C-R210
AAATCTCCTTACATTGGCGGCCCGAAGAGCGGACAACGTCATGTGATCTTCAACGTCA

Last Updated on 06.12.2018 16:11