Sequences from confirmation amplicon


Confirmation sequences for line SALK_050009c and hit At4g03153 are shown.
Line ID SALK_050009c
Locus At4g03153 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1395745
Primers used for PCR BN61 - R204, show primer details
   
Sequence Name 48-K105819-SALK_050009C-BN61
GenBank Accession LN485449 [GenBank]
Sequencing primer gene specific primer, BN61: 5'-TGTTTCCAACATTCCATATTCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K105819-SALK_050009C-BN61
GAGTTTTGACCCCCAAAGAAAAAAAAAGTTGAGATAAAACGAAATCTTTTAGTACATATC
ATCAAAATATCTAAAACGAAAACATTATGAGAAAATAGTAACGAAATCTTTTAGTACATA
TCACCCAACCTCCAATAGGATCATGAGAAAATAGTAAACAAAAACGCAAAAGGTGATAAG
AAAATATTTCCCAATTGAATTGGAAGCAAAAGCAAACCCTAACTAAATTTGCAAGCAACC
GAGTCACTACTTCTTTCTTCTCCTTGTAAACTCTGTTTTCTTCTCTAAGTATCTCCAACT
CTTCCTTCATTGTCTCATCACCATCGTCAAATTCAACAATTTGGTCTTTTTCAGTTTCAG
CTTCAACATCAGACGACTCTGTTTCTTCACACACTTCCGATCGGATCTCGTCGCATGATG
ATGAGTTATGGCTGTCCGAATTCAGAGAAGAGGTTTTGATCAAGAGATCATGCTTCTGCG
CCAAAGAGCGATGTGAACGGTATAATTCCTCGAGCAATTCAATGAGCTTTGGCTTATTCT
CGTAATTAATCTTTGCTCGTTTCATCAAGGAATCTCCTTCATCTTCAACCTCATCAATCA
CGCTCAGCATTTCCTTTGTCTTTGAATCAAGCTCTGTTATTTTATGTTACAACAACCAAA
TTAGTAAGAGAGCTTTTGCGATTAGGGTTAGAACTTGAAAAAACGTAGAAAGATAAGTTT
TTGAGTTACCAGAGAGAGTAGAGTTAAGCCAAGGCGAGGAATTTGATGTGTTGTGATTTG
CACCAATCCACCACCATTTTGATGTCTCACCCATCGTCTTCACTTGTAATTTTGCACCTA
CTTCAAGATTTTTGACAGTTTAATTAATGCAAATAGAGATTTCAAGGGTGACGTATCAAT
CCTCTTTTCTCTCTNTGGCCTCTCTCTCTTTATGGTTTAAGGTGGTGTGTTAGAGCATGT
CCATTGGTTATATTGTGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAAT
A
   
Sequence Name 64-K105820-SALK_050009C-R210
GenBank Accession LN485835 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K105820-SALK_050009C-R210
GTCATTTGTTTACCACAATATATCCACAATATAACCAATGGACATGCTCTAACACACCAC
CTTTAAACCATAAAGAGAGAGAGGCCAAAGAGAGAAAAGAGGATTGATACGTCACCCTTG
AAATCTCTATTTGCATTAATTAAACTGTCAAAAATCTTGAAGTAGGTGCAAAATTACAAG
TGAAGACGATGGGTGAGACATCAAAATGGTGGTGGATTGGTGCAAATCACAACACATCAA
ATTCCTCGCCTTGGCTTAACTCTACTCTCTCTGGTAACTCAAAAACTTATCTTTCTACGT
TTTTTCAAGTTCTAACCCTAATCGCAAAAGCTCTCTTACTAATTTGGTTGTTGTAACATA
AAATAACAGAGCTTGATTCAAAGACAAAGGAAATGCTGAGCGTGATTGATGAGGTTGAAG
ATGAAGGAGATTCCTTGATGAAACGAGCAAAGATTAATTACGAGAATAAGCCAAAGCTCA
TTGAATTGCTCGAGGAATTATACCGTTCACATCGCTCTTTGGCGCAGAAGCATGATCTCT
TGATCAAAACCTCTTCTCTGAATTCGGACAGCCATAACTCATCATCATGCGACGAGATCC
GATCGGAAGTGTGTGAAGAAACAGAGTCGTCTGATGTTGAAGCTGAAACTGAAAAAGACC
AAATTGTTGAATTTGACGATGGTGATGAGACAATGAAGGAAGAGTTGGAGATACTTAGAG
AAGAAAACAGAGTTTACAAGGAGAAGAAAGAAGTAGTGACTCGGTTGCTTGCAAATTTAG
TTAGGGTTTGCTTTTGCTTCCAATTCAATTGGGAAATATTTTCTTATCACCTTTTGCGTT
TTTGTTTACTATTTTCTCATGATCCTATTGGAGGTTGGGTGATATGTACTAAAAGATTTC
GTTACTATTTTCTCATAATGTTTTCGTTTTAGATATTTTGATGAATATGTACTAAAGATT
TCGTTTTATCTCAACTTTTTTTTTCTTTGGGGGTCAAAACTCAAAACTCTTATCTATGTA
CATTTTTTAAACCTTG

Last Updated on 06.12.2018 16:11