Sequences from confirmation amplicon


Confirmation sequences for line SALK_050669c and hit At3g62700 are shown.
Line ID SALK_050669c
Locus At3g62700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23190761
Primers used for PCR 54aq - R204, show primer details
   
Sequence Name 15-K106831-SALK_050669C-R210
GenBank Accession LN484686 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K106831-SALK_050669C-R210
CCACAGTAATTTGTTTCGCATAACCCTTCCTAAACACAGTAATTGTCTTTGACCAACACT
CCAGTTCTCGCCATTATCCGCCACTGTGGCAAATATTCACCAATGTTAATGATCATCTCA
AGTAACATCTGTGAATTAAGTTTCAAAACAAGTAACTTTTAACTCACCGAGAGAGTCGAG
CTTCTCGGGCTTGGAAGCAACAACATCCTTAAGTTGACATCGCTCGAGACTCTATAAACC
AAAGAAATAACAGAACAAATTTTATAAAATGTTGGATCAAGAATCGAATAAAAAAGGTTT
AATCTTTTAAACCTCTAACCTTCCAGATTTCTTCATCAG
   
Sequence Name 21-K106832-SALK_050669C-54aq
GenBank Accession LN484873 [GenBank]
Sequencing primer gene specific primer, 54aq: 5'-TCTGATGAAGAAATCTGGAAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K106832-SALK_050669C-54aq
CCTTTTTATTCGATTCTTGATCCACATTTTATAAAATTTGTTCTGTTATTTCTTTGGTTT
ATAGAGTCTCGAGCGATGTCAACTTAAGGATGTTGTTGCTTCCAAGCCCGAGAAGCTCGA
CTCTCTCGGTGAGTTAAAAGTTACTTGTTTTGAAACTTAATTCACAGATGTTACTTGAGA
TGATCATTAACATTGGTGAATATTTGCCACAGTGGCGGATAATGGCGAGAACTGGAGTGT
TGGTCAAAGACAATTACTGTGTTTAGGAAGGGTTATGCTGAAACAAATTACTGTGGATAA
CACATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAACGGNTCCACG
Primers used for PCR 25au - R212, show primer details
   
Sequence Name 16-K106831-SALK_050669C-R214
GenBank Accession LN484712 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106831-SALK_050669C-R214
GGCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCG
CCTTCAGTTTAAACTATCAGTGTTGCTGAAGTTCCGAGCGCGGCCGTGAGGCTTGGACGC
TAGGGCAGGGCATGAAAAAGCCCGTAGCGGGCTGCTACGGGCGTCTGACGCGGTGGAAAG
GGGGAGGGGATGTTGTCTACATGGCTCTGCTGTAGTGAGTGGGTTGCGCTCCGGCAGCGG
TCCTGATCAATCGTCACCCTTTCTCGGTCCTTCAACGTTCCTGACAACGAGCCTCCTTTT
CGCCAATCCATCGACAATCACCGCGAGTCCCTGCTCGAACGCTGCGTCCGGACCGGCTTC
GTCGAAGGCGTCTATCGCGGCCCGCAACAGCGCAATGTGTTATTAAGTTGTCTAAGCGTC
AATTTGTTAGTAATTTGTTGACAATTACTGTGTTTCTAGACGAAGCAACTGCGTCGGTTG
ATTCGCAGACTGATGCTATGATCCAAAAGATCATCAGGGAGGATTTCTCGGACTGTACCA
TTATCAGCATTGCTCATAGGATTCCAACAGTTATGGACTGTGATCGCGTTCTTGTCATAG
ACGCAGGTAACAAAACAGAACATACAAGTTGTTTACCTTTTTGGTTGATTAGTGGAGTTT
GTAACGGTTTGTGAAATCTGCAGGTAAAGCGAAAGAGTATGATAGTCCAGTCCGTTTACT
GGAGAGACAGTCGTTGTTTGCTGCATTGGTTCAAGAGTATGCTCTCCGATCTGCCGGAAT
ATGAGACCACTTCTTTTTTTTTTGCTTTTCGTTTCTTTTCT
   
Sequence Name 22-K106832-SALK_050669C-25au
GenBank Accession LN484903 [GenBank]
Sequencing primer gene specific primer, 25au: 5'-TCCAAATATCGAGCAGAGAGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106832-SALK_050669C-25au
TATCAAGCATTCGGAAGACAATAGAGCATTGCCAAAGGAATCTTGTGATTATTGAAATCT
TATTAAATCTACATAAACCGTCAATTCTCAACTAAATTATAAAGACAAGGTGTTTTCATA
TTCATATTAGTTGAGTCAACAAAGTTGACTACTAAGAAAAGAAACGAAAAGCAAAAAAAA
AAGAAGTGGTCTCATATTCCGGCAAATCGAAAAGCATACTCTTGAACCAATGCAGCAAAC
AACGACGGTCTCTCCAGTAAACGGACGGGACTATCAAACTCTTTCGCTTTACCTGCAAAT
TTCACAAACCGTTACAAACTCCACTAATCAACCAAAAAGGTAAACAACTTGTATGTTCTG
TTTTGTTACCGGCGTCTATAACAAGAACGCGATCACAGTCCATAACTGTTGGAATCCTAT
GAGCAATGCTGATAATGGTACAGTCCAAGAAATCCTCCCTGATGATCTTTTGGATCAAAG
CATCAGTCTGCAAATCAACCGACGCATTTGCTTCGTCTAGAAACACAGTAATTGTCAACA
AATTACTAACAAATTGACGCTTAAACAACTTAATAACACATTGCGCTGTTGCGGGCCGCG
ATAAACGCCTTCAACAAACCCGGTCCGGACGCAGCGTTCAAGCAGGGACTCGCGGTGATT
GTCAATGGATTGGCGAAAAGGAGGCTCGTTGTCAGGAACGTTGAAGGACCGAGAAAGGGT
GACAATTGATCAGGACCGCTGCCGGAGCGCAACCCACTCACTACAGCAAAGCCATGTAGA
CAACATCCCCTCCCCCTTTCCACCGCGTCAAACGCCCGTAGCAGCCCGCTACGGGCTTTT
TCATGCCCTGCCCTAGCGT

Last Updated on 06.12.2018 16:11