Sequences from confirmation amplicon


Confirmation sequences for line SALK_050811 and hit At2g33340 are shown.
Line ID SALK_050811
Locus At2g33340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14126888
Primers used for PCR 79by - LBb3, show primer details
   
Sequence Name 23-K108444-SALK_050811C-79by
GenBank Accession LN484939 [GenBank]
Sequencing primer gene specific primer, 79by: 5'-CCTGGTCACAATAAAGAAAGGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K108444-SALK_050811C-79by
ATCCTGGAATTGCAAAAGGAATCACGAATCTGATATTCGTGGATAAATGTAAATATTGTG
CCCAGTGATTTTTGACCAAGTACAGTCAAGGGTATTCTTTAACATTTAGTTAAAATTTTG
TTCTCAGAGACCAGCTGAAATCATTTGTTGGAATACGGAATACTAAGCAGACCACCACTG
TCTAGAACAGATTAAATAATACCAAATGCGGTTTAGCACCATCGGTTTGGGATCTTCATT
AGTGTCTGAATTCATTTGGCAACAGAAAGAACACATTTTTTGGTACATATATTGAAGGTA
CAAAGCGATTAAAAGAGCCTATAAGAAAACTCGAGGGTTTGTGAGTGTCATTGTTTGAAT
TCCAAGAGGTAGCGAGGATGATCACAATCACCACTCACTCATGACCCGCACCTATGAAAT
TCTCCCCCATTGCTGCAAATACTGTAAGATACAACAGAACTTGAGAAATTTGAGCGAGAT
AACACTGGAGATGGACATTTGGAATGTTCTGTCTGAGTTTTTTACGGATTGTTCACGAGT
CTTGCGCAGAGTCATCATCGACGTTGGCTTTTTCATCACCAGGAAGACCAAATATCCGTA
GGTTACGGTCCATCGAACCGACTGCAACGTACTGTGCATCTGAACCAAACTTCACACACG
TAGCTTTACCTGCAAGTGTAAGGGCTTCGAAATTAGCAACATTATCGCTATGTGATCATT
GAAGGAGTTAGGTAAGG
   
Sequence Name 07-K108443-SALK_050811C-R210
GenBank Accession LN484450 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K108443-SALK_050811C-R210
GGGTTACCATTTATGTGTTTTGGCTGCAGAGTATACCAGACGGCAAGTGTGAAAGCTGAA
TGGAACCTTATCAAGACACTCCCAGATCTCTCCGGCACTGGTAAAGTCCCTTACCTAACT
CCTTCAATGATCACATAGCGATAATGTTGCTAATTTCGAAGCCCTTACACTTGCAGGTAA
AGCTACGTGTGTGAAGTTTGGTTCAGATGCACAGTACGTTGCAGTCGGTTCGATGGACCG
TAACCTACGGATATTTGGTCTTCCTGGTGATGAAAAAGCCAACGTCGATGATGACTCTGC
GCAAGACTCGTGAACAATCCGTAAAAAACTCAGACAGAACATTCCAAATGTCCATCTCCA
GTGTTATCTCGCTCAAATTTCTCAAGTTCTGTTGTATCTTACAGTATTTGCAGCAATGGG
GGAGAATTTCATAGGTGCGGGTCATGAGTGAGTGGTGATTGTGATCATCCTCGCTACCTC
TTGGAATTCAAACAATGACACTCACAAACCCTCGAGTTTTCTTATAGGCTCTTTTAATCG
CTTTGTACCTTCAATATATGTACCAAAAAATGTGTTCTTTCTGTTGCCAAATGAATTCAG
ACACTAATGAAGATCCCAAACCGATGGTGCTAAACCGCATTTGGTATTATTTAATCTGTT
CTAGACAGTGGTGGTCTGCTTAGTATTCCGTATTCCAACAAATGATTTCAGCTGGTCTCT
GAGAACAAAATTTTAACTAAATGTTAAAGAATACCCTTGACTGTACTTGGTCAAAAATCA
CTGGGCACAATATTTACATTTATCCACGAATATCAGATTCGTGATTCCTTTTGCAATTCC
AGGATTAGTTTAACTTCACGCCAAAGATAATGTTTTGTAATACCCTTTCTT

Last Updated on 06.12.2018 16:11