Sequences from confirmation amplicon


Confirmation sequences for line SALK_050877c and hit At2g45100 are shown.
Line ID SALK_050877c
Locus At2g45100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18595319
Primers used for PCR 02ha - LBb3, show primer details
   
Sequence Name 17-K112064-SALK_050877C-02ha
GenBank Accession LN484746 [GenBank]
Sequencing primer gene specific primer, 02ha: 5'-GTTTCTTTTGTAGAAGGAAGCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K112064-SALK_050877C-02ha
GTATGCTGCATATGCAGCAACATTTTCGAAGCTTCTAAGCAGCTGCGGCGAAATCTAGAA
AAGAAAAACGACAACAACGCGCTGAGGAAGAAAAGAACGCTCCTCCTCCAGCTACAGGCA
TAGAAGCTGTTGATAGCATGGTCAAGAGAAAGGTAAAAAAAACATTGTATATTTTGAAGA
AAAATAATTCTTTTGTGTTTCTGATTTTGAGTCTTTAACTTCTTATACAGAAATTTCGCG
ATATTAACTGCGATTATTTGGAGGAGCTTTTTGATGCGTCAGTTGAGAAATCACCCAAGA
GATCGAAAACCGAGACAGTGATGGAGAAGAAGAAGAAGGAAGAGCATGAGATTGTTGAAA
ACGAACAAGAGGAAGAAGACTACGCGGCACCATACGAACAAGATGAAGAAGACTACGCGG
CACCATACGAGATGAACACAGATNAGAAATTCTATGAAAGCGAAGTAGAAGAGGAAGAGG
ATGGTTACGATTTTGGATTGTATTAAAATCATACTTTTTATCATCTAATTATTATATTGT
GGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTG
TTTGATGGTGGTTCCG
   
Sequence Name 01-K112049-SALK_050877C-R210
GenBank Accession LN484277 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112049-SALK_050877C-R210
TTTGTTTACCACAATATAATAATTAGATGATAAAAAGTATGATTTTAATACAATCCAAAA
TCGTAACCATCCTCTTCCTCTTCTACTTCGCTTTCATAGAATTTCTTATCTGTGTTCATC
TCGTATGGTGCCGCGTAGTCTTCTTCATCTTGTTCGTATGGTGCCGCGTAGTCTTCTTCC
TCTTGTTCGTTTTCAACAATCTCATGCTCTTCCTTCTTCTTCTTCTCCATCACTGTCTCG
GTTTTCGATCTCTTGGGTGATTTCTCAACTGACGCATCAAAAAGCTCCTCCAAATAATCG
CAGTTAATATCGCGAAATTTCTGTATAAGAAGTTAAAGACTCAAAATCAGAAACACAAAA
GAATTATTTTTCTTCAAAATATACAATGTTTTTTTTACCTTTCTCTTGACCATGCTATCA
ACAGCTTCTATGCCTGTAGCTGGAGGAGGAGCGTTCTTTTCTTCCTCAGCGCGTTGTTGT
CGTTTTTCTTTTCTAGATTTCGCCGCAGCTGCTTTAGAAGCTTCGAAAATGTTGCTTGCA
TTATTGCAAGCATTTAAACCTGCTGCTTTCTTAGCTGCTTCCTTCTA

Last Updated on 06.12.2018 16:11