Sequences from confirmation amplicon


Confirmation sequences for line SALK_051124c and hit At3g14990 are shown.
Line ID SALK_051124c
Locus At3g14990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5047351
Primers used for PCR 25ea - LBb3, show primer details
   
Sequence Name 64-K110715-SALK_051124C-25ea
GenBank Accession LN485843 [GenBank]
Sequencing primer gene specific primer, 25ea: 5'-TCCACTTATTGGCTGTCCTTAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K110715-SALK_051124C-25ea
ACAATGGACATGTTAAATCAGCAGAGAGACTTACGGGGAGCACAATAAGGTCGAAAACAG
AGTCGGTAATATCAGAGAGAAGAGTATCAGCGACCATCTTGATGCCATGACAAGCATCAA
CGCCAACCTGAGTCTCAACAGACGCCACCGTCACGTCAGCACCACCTCGACGCAACACGG
TAATCATCGCCACCGCTTCTAGAGGCTCCGTACCGTGCGCAATAGGGATCAAAACCTAAA
AGAGACGCCAAAAAACCTTGGATTAATAATCACAAAAAAAAATCGTTACGTACGAGGAGG
GAAAAAAGTATGATCTCGGGATCGTGAAACCCCACACACCGTTTTCGTAAATGAAGCCAT
AGTTGCGGAGATTAGAGAAACGATGGTCGAGTCAAGAAGAAAGAGAAATGGAGTGTAGTG
GAGAAGAAAGGAGTGTCACTAATGGGGTTAAAATAGTAGGAAAGACTCAGTGACGAAGCC
ATTTACGACTTTCCTTTTTATCACTTTAACCAAAAGAAGATAGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 48-K110714-SALK_051124C-R210
GenBank Accession LN485455 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K110714-SALK_051124C-R210
CTTCTTTTGGTTAAGTGATAAAAAGGAAAGTCGTAAATGGCTTCGTCACTGAGTCTTTCC
TACTATTTTAACCCCATTAGTGACACTCCTTTCTTCTCCACTACACTCCATTTCTCTTTC
TTCTTGACTCGACCATCGTTTCTCTAATCTCCGCAACTATGGCTTCATTTACGAAAACGG
TGTGTGGGGTTTCACGATCCCGAGATCATACTTTTTTCCCTCCTCGTACGTAACGATTTT
TTTTTGTGATTATTAATCCAAGGTTTTTTGGCGTCTCTTTTAGGTTTTGATCCCTATTGC
GCACGGTACGGAGCCTCTAGAAGCGGTGGCGATGATTACCGTGTTGCGTCGAGGTGGTGC
TGACGTGACGGTGGCGTCTGTTGAGACTCAGGTTGGCGTTGATGCTTGTCATGGCATCAA
GATGGTCGCTGATACTCTTCTCTCTGATATTACCGACTCTGTTTTCGACCTTATTGTGCT
CCCCGTAAGTCTCTCTGCTGATTTAACATTGTCCATTTGTTTTGTTTCTTTGTGTGTAAT
CTAAGAACTAAGGACAGCCAATAAGTGG

Last Updated on 06.12.2018 16:11