Sequences from confirmation amplicon


Confirmation sequences for line SALK_051647c and hit At2g38090 are shown.
Line ID SALK_051647c
Locus At2g38090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15945818
Primers used for PCR 74hw - LBb3, show primer details
   
Sequence Name 11-K113018-SALK_051647C-74hw
GenBank Accession LN484586 [GenBank]
Sequencing primer gene specific primer, 74hw: 5'-TCTCCATTTCTCTGTTTGGTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K113018-SALK_051647C-74hw
TCTTCTCTGTTTGCTTTCATTTTCTCTGGATTCGGATCTGACCACTAAAGAGAGGTTTAT
CGTTTCAGACAACAAAACTCAAAGATCGAATCTATCTTTTGTCCTTAGAGTCTGGAAAAA
GCAGCTCTCTTTTGATCTCCTCTTTAGATTCTTCAAAGCTTTAGATGCTTATCTCTCTTT
CTCTCTCAGATTCCATTTGAATAAATGCCATTTTGACAGATCAAATATCTCCCACTTTTC
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGAGCTCCACCATTATCGAAAACTCTGT
TTTTTTCTTTCTTTCGTGATTCGATCAACAATCGTTGAAAACATGAACAGAGGAATCGAA
GTTATGTCACCAGCAACATATCTAGAGACATCAAACTGGTTGTTTCAAGAAAACAGAGGA
ACCAAATGGACAGCTGAAGAAAACAAGAAATTCGAAAACGCTTTAGCCTTTTACGACAAA
GATACTCCCGACAGATGGTCCAGAGTCGCTGCCATGCTTCCCGGTAAAACAGTCAGAGAT
GTGATCAAACAATACAGAGAGCTTGAGGAAGACGTTAGCGACATCGAAGCTGGTCTTATA
CCAATCCCTGGTTACGCCTCTGATTCATTCACACTTGATTGGGGTGGCTACGACAGAGCA
AGCGGTAACAATGGGTTTAACATGAACGGTTACTACTTTTCCGCCGCCAGAAGAGAGAGA
GGATCCGCCGCACGAACGGCGGAGCATGAGAGGAAGAAATGTGTTCCATGGACAGAAGAA
GAACACGGGTAAACTTAAATCATCTCTCTACGTTAACGTCAGTGCTTTTCTTCTCTAGAT
GTGGACCCCTATACACTTTCTTATATAAAAATACGTACGTCTTATAACACAGTGCGGAAC
ACTTAATAACACATTGCGGACGTTTTTAGTGTACTGGGGTGGTT
   
Sequence Name 03-K113017-SALK_051647C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K113017-SALK_051647C-R210
ACTGAGTGTCTCGGGTTTTGAAAACATACGCTCTTGAGGGTGG

Last Updated on 06.12.2018 16:11