Sequences from confirmation amplicon


Confirmation sequences for line SALK_051729 and hit At4g24970 are shown.
Line ID SALK_051729
Locus At4g24970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12833173
Primers used for PCR 56us - LBb3, show primer details
   
Sequence Name 22-K117668-SALK_051729C-R210
GenBank Accession LN484927 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117668-SALK_051729C-R210
CATTTGTTTACCACAATATATCCTGTGACAGAATAAAAGAAGATCAAATAGCTCAGTCGC
TCTCATGCAAAATTTCTTAGTATGAATAAGTGATTAAGCAACTGCTTTGAACAAAGGGAA
AATATACCTGATGAAGAAGATCTTCTTCACTAGAGAATGGCGACCATTGAATTATTGTTT
CCACATTTTTATCCCAATCTCTCGTGGAAGACCGGATTATTTTACTCCATTCAGGGTCCC
TTCTTTCATAGTCAAGCTGTCATTTTAAATCACATAACAAGAGAATATAAGGATATAATG
AATACAAAATTCTTTTAAATTAGAAAGTATACGTTGTGGACATAAGACATATTAGTGCTT
CACACTTCCTCTAACACTGAACAAAGATTGCAATAAATAGTGTACACGTAACCAGCTGAT
ACGTGCCACTGAATCCCATGTTNACCAAAGAT
   
Sequence Name 59-K117669-SALK_051729C-56us
GenBank Accession LN485724 [GenBank]
Sequencing primer gene specific primer, 56us: 5'-ATCTTTGGTTAACATGGGATTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K117669-SALK_051729C-56us
TGCATCTTTGTTCAGTGTTAGAGGAAGTGTGAAGCACTAATATGTCTTATGTCCACAACG
TATACTTTCTAATTTAAAAGAATTTTGTATTCATTATATCCTTATATTCTCTTGTTATGT
GATTTAAAATGACAGCTTGACTATGAAAGAAGGGACCCTGAATGGAGTAAAATAATCCGG
TCTTCCACGAGAGATTGGGATAAAAATGTGGAAACAATAATTCAATGGTCGCCATTCTCT
AGTGAAGAAGATCTTCTTCATCAGGTATATTTTCCCTTTGTTCAAAGCAGTTGCTTAATC
ACTTATTCATACTAAGAAATTTTGCATGAGAGCGTCTAAGCTATTTGATCTTCTTTTATT
CTGTCACAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11