Sequences from confirmation amplicon


Confirmation sequences for line SALK_052789c and hit At2g45760 are shown.
Line ID SALK_052789c
Locus At2g45760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18847551
Primers used for PCR 45fp - LBb3, show primer details
   
Sequence Name 13-K111624-SALK_052789C-45fp
GenBank Accession LN484637 [GenBank]
Sequencing primer gene specific primer, 45fp: 5'-GATCGACCACGTCATTATTCCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K111624-SALK_052789C-45fp
ATGGAATTAATTTTATGTAGAAATCAAAATTCAGAATAAAAATATATACCCNGGTCCGTT
ACGTGATAAGAAAAGAATATATACCCNGTTAGGATTAAAAAATTTAAAATTGTACAAACG
GTCTAGTGTGAGAAAAATACAATTTAAACCAAAAACAAAATGTGAGAAAATAAATTTTGT
TTGCACTTTCCGAGAAAAAAAGATACTTAGAACAAAAAAAATAATAGAAAGTGACTGGAA
ACAACTACGAAAAGGGACGGTGTTCCTCAGTCAATTTAGCTAAGCTATATAATATAAACT
CATCCTCTCTAACAAATCTCACGAAACGAAACAATAAGGAATCTCCTGCAAAGAGGATTA
GACTTATACATATAGAAACATAAAACACACAAAACACACCATGTCGTATTCAACATTCAA
AAGATCACTGGAGATTGAGGTAATCTCAGCGGAAGGACTCAAGGTAGATCGAAAACCATT
GAAGAAGAAAACATACAGCGTTGTGAGAATCGATGAGAAATCTTGGGCTTCCAAAGTGGA
TGAACTAGGAGGGAGTTATCCAATTTGGAAAGATAGATTTGATATGGAGATGCCAATAAC
AAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGA
GAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT
GGT
   
Sequence Name 05-K111623-SALK_052789C-R210
GenBank Accession LN484398 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K111623-SALK_052789C-R210
CGTCATTTGTATTGGCATCTCCATATCAAATCTATCTTTCCAATTGGATAACTCCCTCCT
ATTTCATCCACTTTGGAAGCCCAAGATTTCTCATCGATTCTCACAACGCTGTATGTTTTC
TTCTTCAATGGTTTTCGATCTACCTTGAGTCCTTCCGCTGAGATTACCTCAATCTCCAGT
GATCTTTTGAATGTTGAATACGACATGGTGTGTTTTGTGTGTTTTATGTTTCTATATGTA
TAAGTCTAATCCTCTTTGCAGGAGATTCCTTATTGTTTCGTTTCGTGAGATTTGTTAGAG
AGGATGAGTTTATATTATATAGCTTAGCTAAATTGACTGAGGAACACCGTCCCTTTTCGT
AGTTGTTTCCAGTCACTTTCTATTATTTTTTTTGTTCTAAGTATCTTTTTTTCTCGGAAA
GTGCAAACAAAATTTATTTTCTCACATTTTGTTTTTGGTTTAAATTGTATTTTTCTCACA
CTAGACCGTTTGTACAATTTTAAATTTTTTAATCCTAACGGGTATATATTCTTTTCTTAT
CACGTAACGGACCGGGTATATATTTTTATTCTGAATTTTGATTTCTACATAAAATATAAT
TCCATGCTTTTCTCAGGGGATGATGGAATAAAATAGATAGGAATAATGACGTGGTCGAT

Last Updated on 06.12.2018 16:11