Sequences from confirmation amplicon


Confirmation sequences for line SALK_052963c and hit At3g22260 are shown.
Line ID SALK_052963c
Locus At3g22260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7872107
Primers used for PCR 14ii - LBb3, show primer details
   
Sequence Name 34-K112623-SALK_052963C-14ii
GenBank Accession LN485202 [GenBank]
Sequencing primer gene specific primer, 14ii: 5'-TGATCTTTGATCTGGGTTTCTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K112623-SALK_052963C-14ii
GTGTCTCTTTTGTAAGATTACAACATTTTAGGTTGATATACCTTTTGGATTTGGTTGCTT
AATGGTATTGGATTGATACTAGTTTCTCAAGATTATGGTTTTCGTTTTAAACTGATCAGA
GGTAGTCTCTGTCTTGCTTCCTAGGTTGTATTTAGTATAGGATCCAAATACAATGGATGA
AAACCATAGGAATCCATTTGCAAATGCAAGTACAAGTGCTAGAGCAAGCGGCAGTACAAG
TGCGAGCTCAAACTCGAGTTTTAGTAGCAGTGTGGCGGATACAGATGATGATCAGACCAT
TGCACGTATATTAGCTGAAGATGAGAGCTTGAGAAGAGAAGGCAAGCTTGGGAAGAGATT
ATCTCATTTGGATTCTATCCCAGTATGATAAGCTTCTTTTGCTTAATCTTCACATTTAGG
CTTGGATCTGTGCTATTCATTGTTGTTGTTAGTGTCTATTGGTGACAAGTTCTTTGAATA
TCTTGTGGAATTTTTTATTGGCAGCACACTCCTCGGGTTAACAGGGAGATACCTGATATA
AATGATGCAACATTAGACCATGAGCTGCTCTCTGGGAGGTTCTCATCTTCTTATCAACGT
TTCCTTTAGTGTCATCGTCTTTCATTTCCTTGACTGCATTCTTCATTTGATGCTGATTAG
ATTGTTCATTGTCGATAGAATAGCTAGTTGTTTTCAATCTAGAAACATCTTTAATGGTCT
CTCTTTTTTGTTCAGGTTGGCCACATATGGTTTAGCGGAATTGCAAATGGAGGGAGATGG
AAATTGCCAGGTGTAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGNGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CCCGCCTGGCCCTGAGAGAGTTTGCAGCAGCGGTCCACGGCTGGTTGCCCCAGCAGGCGA
AAATCCTGTTTGA
   
Sequence Name 10-K112622-SALK_052963C-R210
GenBank Accession LN484552 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K112622-SALK_052963C-R210
GTCATTTGTTTACCCTGGCAATTTCCATCTCCCTCCATTTGCAATTCCGCTAAACCATAT
GTGGCCAACCTGAACAAAAAAGAGAGACCATTAAAGATGTTTCTAGATTGAAAACAACTA
GCTATTCTATCGACAATGAACAATCTAATCAGCATCAAATGAAGAATGCAGTCAAGGAAA
TGAAAGACGATGACACTAAAGGAAACGTTGATAAGAAGATGAGAACCTCCCAGAGAGCAG
CTCATGGTCTAATGTTGCATCATTTATATCAGGTATCTCCCTGTTAACCCGAGGAGTGTG
CTGCCAATAAAAAATTCCACAAGATATTCAAAGAACTTGTCACCAATAGACACTAACAAC
AACAATGAATAGCACAGATCCAAGCCTAAATGTGAAGATTAAGCAAAAGAAGCTTATCAT
ACTGGGATAGAATCCAAATGAGATAATCTCTTCCCAAGCTTGCCTTCTCTTCTCAAGCTC
TCATCTTCAGCTAATATACGTGCAATGGTCTGATCATCATCTGTATCCGCCACACTGCTA
CTAAAACTCGAGTTTGAGCTCGCACTTGTACTGCCGCTTGCTCTAGCACTTGTACTTGCA
TTTGCAAATGGATTCCTATGGTTTTCATCCATTGTATTTGGATCCTATACTAAATACAAC
CTAGGAAGCAAGACAGAGACTACCTCTGATCAGTTTAAAACGAAAACCATAATCTTGAGA
AACTAGTATCAATCCAATACCATTAAGCAACCAAATCCAAAAGGTATATCAACCTAAAAT
GTTGTAATCTGTAACAAAAGAAGACAACTTTCGATTCAATCACACAAAACTACAGAAACC
CAGATNNAAGATC

Last Updated on 06.12.2018 16:11