Sequences from confirmation amplicon


Confirmation sequences for line SALK_053439c and hit At2g04025 are shown.
Line ID SALK_053439c
Locus At2g04025 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1277493
Primers used for PCR 69sa - LBb3, show primer details
   
Sequence Name 47-K116392-SALK_053439C-69sa
GenBank Accession LN485440 [GenBank]
Sequencing primer gene specific primer, 69sa: 5'-TATTTGAATATGGACGTTATGGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K116392-SALK_053439C-69sa
ACATTACACGTTAACAGAGACACATGGTCAAAGACTAATTATAGATACACGAACTTAAAA
CATATGGCCACTAATCTCCTATATATAGACGCATCTCTTGCTTTTAATAATTCTACCTCC
TGCTTAAATTGTAAGAAAATATTATCAGAAAAATAAAGGAAGATATCAAGTTTTGAAGGA
GAATATAAAAGAGTCATGACTACTTTGTCAAAGATTCTATGTGTGTTGATCATTCTTCTT
TTATGTTTCTCCTTTCGCTATTCACTTCATGGTTTGTGTGTTTCTCTCTCCTCACAGTAT
CTTCTTTTGCATCTAAAAGAGCAAAATCATAAAGCCTATATAGTCTCTAACTTTTATTTT
ACTTTTAGTTGCATGCATAATCACAGGATATATTGTGGTGTAAACAAATTGACGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCG
GTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 22-K116391-SALK_053439C-R210
GenBank Accession LN484922 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116391-SALK_053439C-R210
CACAATATATCCTGGTGATTATGCATGCAACTAAAAGTAAAATAAAAGTTAGAGACTATA
TAGGCTTTATGATTTTGCTCTTTTAGATGCAAAAGAAGATACTGTGAGGAGAGAGAAACA
CACAAACCATGAAGTGAATAGCGAAAGGAGAAACATAAAAGAAGAATGATCAACACACAT
AGAATCTTTGACAAAGTAGTCATGACTCTTTTATATTCTCCTTCAAAACTTGATATCTTC
CTTTATTTTTCTGATAATATTTTCTTACAATTTAAGCAGGAGGTAGAATTATTAAAAGCA
AGAGATGCGTCTATATATAGGAGATTAGTGGCCATATGTTTTAAGTTCGTGTATCTATAA
TTAGTCTTTGACCATGTGTCTCTGTTAACGTGTAATGTATGAATTCTTACGATGAAAAGT
TGTACTTGTAAATATTTACGATTCCTGCCATAACGT

Last Updated on 06.12.2018 16:11