Sequences from confirmation amplicon


Confirmation sequences for line SALK_053649c and hit At5g51690 are shown.
Line ID SALK_053649c
Locus At5g51690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21000973
Primers used for PCR 21md - LBb3, show primer details
   
Sequence Name 30-K115696-SALK_053649C-21md
GenBank Accession LN485134 [GenBank]
Sequencing primer gene specific primer, 21md: 5'-GTTCAATCATTTTGTGACCCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K115696-SALK_053649C-21md
TTTTCTTTGTGTGTGGAATGTTGCGATGTTCACGTGAAGGAGTTCGACGAGTTCATGGAA
ATACCTCCTTGCACTAAAGGATGGCATAGCAGCAGCCCTGATCCGGCGGTCTGATATATT
TTTTTTCCTGAGATTTTCGATTCAAAGATTAATCATCTTTTCTAATTGTGTTTTCATGTT
CTTCTTATCATATGGAATTCTATTTTAGATTTGTTATAGAACCTTTCAGTTGACAGATAT
ATGTAATGGTTAAATGCTCATCTTCTGTGTTTTGGATCATGATTATGGCTTTGATTGTTG
TTTTCAAAACTTTGAAGATCTTTAAATAATTTCACCAATATTAGAAGGACTATTTAGCAA
CTTTTAAAACGTAGAGACAAATTGCGAATATGGAAAAATAATTTGCCAATTTATCTCAAA
ACTTTTGTATTTATTCGACTCTTTTTCAACTTCGTTACTCTCAACGAACGGCTCGAATCT
CTCTTCAACGCCGGAGGCAACAACTATCTCCGCGGACAACTTAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAG
CTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG
CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 19-K115695-SALK_053649C-R210
GenBank Accession LN484824 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115695-SALK_053649C-R210
TCGCGGAGATGTTGTTGCCTCCGGCGTTGAAGAGAGATTCGAGCCGTTCGTTGAGAGTAA
CGAAGTTGAAAAAGAGTCGAATAAATACAAAAGTTTTGAGATAAATTGGCAAATTATTTT
TCCATATTCGCAATTTGTCTCTACGTTTTAAAAGTTGCTAAATAGTCCTTCTAATATTGG
TGAAATTATTTAAAGATCTTCAAAGTTTTGAAAACAACAATCAAAGCCATAATCATGATC
CAAAACACAGAAGATGAGCATTTAACCATTACATATATCTGTCAACTGAAAGGTTCTATA
ACAAATCTAAAATAGAATTCCATATGATAAGAAGAACATGAAAACACAATTAGAAAAGAT
GATTAATCTTTGAATCGAAAATCTCAGGAAAAAAAATATATCAGACCGCCGGATCAGGGC
TGCTGCTATGCCATCCTTTAGTGCAAGGAGGTATTTCCATGAACTCGTCGAACTCCTTCA
CGTGAACATCGCAACATTTCCACTACACAAAGAAAACAGTGATATTCAATAGAAATTCAA
ACAGTGAAAGAAAAGGGTCA

Last Updated on 06.12.2018 16:11