Sequences from confirmation amplicon


Confirmation sequences for line SALK_054291 and hit At1g78920 are shown.
Line ID SALK_054291
Locus At1g78920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29673810
Primers used for PCR 52dl - LBb3, show primer details
   
Sequence Name 53-K110195-SALK_054291C-52dl
GenBank Accession LN485578 [GenBank]
Sequencing primer gene specific primer, 52dl: 5'-TTTTCTCGGTATGAGTCAGGAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K110195-SALK_054291C-52dl
AGTACTTTAAGAGAGATGGTGCACCTTCAATTTTGCACTTCTTAGCCATTGTTCCACCTA
GTATCATTGCACTGATGATTTCTGCAGCTATACTTTCAAATAAATCAGCACCTCTAGCAG
CACAGTCCCCCACATTGTCTCCAACCTACAAGAAGATGAAAATGGCTTTAAATGCCTAAA
TGACGAAAACACAAGCAAACCCTTGTAGAGTGATATATGCCCCAAACAAGGATGTCACGT
TTGGGAATCACAGAATCAAGAAAGTAAGAAACAAAAAGAACAAAGATCAAATACCAAATC
AGCAATAACAGCAGGATTTCGAGGATCATCCTCGGGAATTCCCTGCTCAACTTTCCCAAC
GAGATCTGCCCCAACATCAGCTCCCTTAGTATATATTCCACCACCCAACTGAGCAAATAA
GGCAACAAACGAAGCACCAAAACCATATCCGACAAGAAGAAGAGGTACTGTAAGAGAAAC
AGAAAATTCAATCAATAAATCTTACATTAATGGGGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 45-K110194-SALK_054291C-R210
GenBank Accession LN485389 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K110194-SALK_054291C-R210
TATGTAGATTATTGATTGAATTTTCTGTTTCTCTTACGTACCTCTTCTTCTTGTCGGATA
TGGTTTTGGTGCTTCGTTTGTTGCCTTATTTGCTCAGTTGGGTGGTGGAATATATACTAA
GGGAGCTGATGTTGGGGCAGATCTCGTTGGGAAAGTTGAGCAGGGAATTCCCGAGGATGA
TCCTCGAAATCCTGCTGTTATTGCTGATTTGGTATTTGATCTTTGTTCTTTTTGTTTCTT
ACTTTCTTGATTCTGTGATTCCCAAACGTGACATCCTTGTTTGGGGCATATATCACTCTA
CAAGGGTTTGCTTGTGTTTTCGTCATTTAGGCATTTAAAGCCATTTTCATCTTCTTGTAG
GTTGGAGACAATGTGGGGGACTGTGCTGCTAGAGGTGCTGATTTATTTGAAAGTATAGCT
GCAGAAATCATCAGTGCAATGATACTAGGTGGAACAATGGCTAAGAAGTGCAAAATTGAA
GGTGCACCATCTCTCTTTAAAGTTACTTCTGGTATATGCAAATATATAACTTATGGTGTG
AGTTCCTGACTCATACCGAGAAA

Last Updated on 06.12.2018 16:11