Sequences from confirmation amplicon


Confirmation sequences for line SALK_054337c and hit At1g53730 are shown.
Line ID SALK_054337c
Locus At1g53730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20064513
Primers used for PCR BL03 - R204, show primer details
   
Sequence Name 36-K104740-SALK_054337C-BL03
GenBank Accession LN485230 [GenBank]
Sequencing primer gene specific primer, BL03: 5'-TCTCCCAGTTAAAAGTTCAAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104740-SALK_054337C-BL03
TCTTCAAGAAATTGGCCTGACATTGATACTTCAGGTGCGCTATACCCTCCATCGGTTTGG
TTTAATAACTGCACATATCAGAATGATCTAAGCAACCAAATAAGTTGAGAAATGGTAAAG
AGTTTTGAGAAACGTTTGTATATCCAACCTCATTAGCTGTGGGAAGGAAGCTTGCGAGAC
CAGAATCTGATAAGTGAGGATTCAGCTCTGAATCGAGTAAAATATTAGCTGATTTGATAT
TCTTGTCCACTATAGACGGTGAGCAAACTTCATGCAGATACCTGCAAGAACAATACTTTC
AGTTAACCAAAATACTCGTTTCTTTTTTAATAGTGAGCTAAGCAATGAATTGTTTATGGA
ATTTACTCCAATGCGCGTGCAGTCCCAAGAGCGATCTTGACGCTTAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
   
Sequence Name 94-K104792-SALK_054337C-R210
GenBank Accession LN486355 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K104792-SALK_054337C-R210
GTCAGATCGCTCTTGGGACTGCACGCGCATTGGAGTAAATTCCATAAACAATTCATTGCT
TAGCTCACTATTAAAAAAGAAACGAGTATTTTGGTTAACTGAAAGTATTGTTCTTGCAGG
TATCTGCATGAAGTTTGCTCACCGTCTATAGTGGACAAGAATATCAAATCAGCTAATATT
TTACTCGATTCAGAGCTGAATCCTCACTTATCAGATTCTGGTCTCGCAAGCTTCCTTCCC
ACAGCTAATGAGGTTGGATATACAAACGTTTCTCAAAACTCTTTACCATTTCTCAACTTA
TTTGGTTGCTTAGATCATTCTGATATGTGCAGTTATTAAACCAAACCGATGAAGGGTATA
GCGCACCTGAAGTATCAATGTCAGGCCAATATTCTTTGAAGAGTGACATTTACAGTTTTG
GAGTAGTGATGCTTGAACTTTTAACTGGGAGA
Primers used for PCR BL04 - LBb3, show primer details
   
Sequence Name 52-K119290-SALK_054337C-BL04
GenBank Accession LN485569 [GenBank]
Sequencing primer gene specific primer, BL04: 5'-ACTGGCAGTTTCAGCGTAGATAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K119290-SALK_054337C-BL04
CAGAGCTGAGTTTGATGATGGAAAGGTAAGATATTATAATATTGTTATGTAATCCTAATC
TCTATGTCTCGAATGATGTCATAATGTTGTTGGAAAACGTCTTATCTTTTAGGTTCTTGC
TGTGAAGAAAATTGATTCGTCTGCTCTCCCACATGGCATGACCGATGATTTCATTGAAAT
GGTATCGAAAATAGCCAATTTGGATCATCCAAATGTGACCAAGCTTGTTGGTTACTGTGC
TGAGCACGGACAACATCTCGTGGTCTATGAATTCCACAAAAATGGATCATTACATGACTT
CTTACACTTATCAGAAGAGGAAAGTAAAGCATTGGGACAACTTAATAACACATTGCGGAC
GTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGC
CCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCA
GGCGAAAATCCTGTTTGATGGTGGTTCCGAAACAGGCAAAA
   
Sequence Name 04-K119289-SALK_054337C-R210
GenBank Accession LN484389 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119289-SALK_054337C-R210
TTACTTTCCTCTTCTGATAAGTGTAAGAAGTCATGTAATGATCCATTTTTGTGGAATTCA
TAGACCACGAGATGTTGTCCGTGCTCAGCACAGTAACCAACAAGCTTGGTCACATTTGGA
TGATCCAAATTGGCTATTTTCGATACCATTTCAATGAAATCATCGGTCATGCCATGTGGG
AGAGCAGACGAATCAATTTTCTTCACAGCAAGAACCTAAAAGATAAGACGTTTTCCAACA
ACATTATGACATCATTCGAGACATAGAGATTAGGATTACATAACAATATTATAATATCTT
ACCTTTCCATCATCAAACTCAGCTCTGTATACTCGTCCAAAAGTCCCTTCTCCAAGAAGA
TTATCTACGCTGAAACTGCCAG

Last Updated on 06.12.2018 16:11