Sequences from confirmation amplicon


Confirmation sequences for line SALK_054774c and hit At5g65920 are shown.
Line ID SALK_054774c
Locus At5g65920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26364692
Primers used for PCR 23ty - LBb3, show primer details
   
Sequence Name 35-K117180-SALK_054774C-23ty
GenBank Accession LN485224 [GenBank]
Sequencing primer gene specific primer, 23ty: 5'-TAGAATTCAACACATCGAAACTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117180-SALK_054774C-23ty
TTCATATACATCAACAAGATGGGGCAGATCCGTTGTGTGATTTTGACCCGAAAGGTGAAA
ACTTGATCAGTCTTTTATTGGATAGTTCTTGTGAGTTTACATTTGGAGATAAACATTGAG
TCTGAATAATGTAGACTACATAACTTCAATAGCTCAGCTGAACGTTGCTTCAAAGCTGGA
TCACATCCACTTTGTATCACAAGCAAAAGCTTTGCAGCCAAACCAACTTCAACAGCAAGA
GATGAACATTCTTCAGAAGCTAATTTGCAAACTGACCAAAGAATCGATATCGCATAGTTT
GTGCATTTCTCTGATACCTTCATCAGTAACCTAACGGTATGCGGAATCGTGTTAACCGAA
TCTTTCAAAGCAATTCTGCCTTCGCTTTCTAAACATAACGAATCCAACACGAAAAGAGCT
GACTCTAAACACTCCACATCCAAACATGGACAAATTGACGCTTAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT
GCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 14-K117179-SALK_054774C-R210
GenBank Accession LN484678 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117179-SALK_054774C-R210
TTTGGATGTGGAGTGTTTAGAGTCAGCTCTTTTCGTGTTGGATTCGTTATGTTTAGAAAG
CGAAGGCAGAATTGCTTTGAAAGATTCGGTTAACACGATTCCGCATACCGTTAGGTTACT
GATGAAGGTATCAGAGAAATGCACAAACTATGCGATATCGATTCTTTGGTCAGTTTGCAA
ATTAGCTTCTGAAGAATGTTCATCTCTTGCTGTTGAAGTTGGTTTGGCTGCAAAGCTTTT
GCTTGTGATACAAAGTGGATGTGATCCAGCTTTGAAGCAACGTTCAGCTGAGCTATTGAA
GTTATGTAGTCTACATTATTCAGACTCAATGTTTATCTCCAAATGTAAACTCACAAGAAC
TATCCAATAAAAGACTGATCAAGTTTTCACCTTTCGGGTCAAAATCACACAACGGATCTG
CCCCATCTTGTTGATGTATATAGAAATGAAGAAGCTCAAGGAGTATGAGAGTAAGAAACG
AGTTTCGATGTGTGAAATTCTA

Last Updated on 06.12.2018 16:11