Sequences from confirmation amplicon


Confirmation sequences for line SALK_055262c and hit At5g49180 are shown.
Line ID SALK_055262c
Locus At5g49180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19942336
Primers used for PCR 11gj - LBb3, show primer details
   
Sequence Name 22-K112064-SALK_055262C-11gj
GenBank Accession LN484914 [GenBank]
Sequencing primer gene specific primer, 11gj: 5'-GGTGAACTGAAAAAGAAGAAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K112064-SALK_055262C-11gj
GTTGTCGCTGTTGGCATCACAACATCAAGAAACACCAGTCATTCAGAAAAAATCGTCCCT
GTGCAGATTAAAACAGCCACCACGGCAGTTGAAGCAGTTTGTGCACCTACTGATTACAAA
GAGACTTGTGTCAATAGTCTCATGAAAGCTTCTCCTGACTCTACTCAGCCTCTTGATCTC
ATTAAGCTTGGCTTCAACGTCACCATTCGATCCATAGAAGATAGCATCAAGAAAGCTTCC
GTGGAGCTGACAGCCAAGGCAGCTAATGACAAGGATACCAAAGGGGCTTTGGAGTTGTGT
GAGAAGCTTATGAATGATGCTACAGATGATCTGAAGAAGTGTCTTGATAACTTTGATGGG
TTCTCAATTCCTCAGATTGAGGACTTTGTCGAAGATCTTCGTGTTTGGCTTAGTGGCTCC
ATTGCTTATCAACAAACATGTATGGATACGTTTGAAGAAACTAACTATTGACGCTTAGAC
AACTTAATAACACATTGGTGTAACAGTTAGCGTCAATTAAACTATCAGTTAGCGTCAATT
AAATAAATTGGTGTCTCCAGGATATATTGTGGTGTAAACAAATTGACGCTNAGACAACTT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
GCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 06-K112049-SALK_055262C-R210
GenBank Accession LN484429 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K112049-SALK_055262C-R210
GTCATTTGTTACACCACAATATATCCTGGAGACACCAATTTATTTAATTGACGCTAACTG
ATAGTTTAATTGACGCTAACTGTTACACCAATGTGTTATTAAGTTGTCTAAGCGTCAATA
GTTAGTTTCTTCAAACGTATCCATACATGTTTGTTGATAAGCAATGGAGCCACTAAGCCA
AACACGAAGATCTTCGACAAAGTCCTCAATCTGAGGAATTGAGAACCCATCAAAGTTATC
AAGACACTTCTTCAGATCATCTGTAGCATCATTCATAAGCTTCTCACACAACTCCAAAGC
CCCTTTGGTATCCTTGTCATTAGCTGCCTTGGCTGTCAGCTCCACGGAAGCTTTCTTGAT
GCTATCTTCTATGGATCGAATGGTGACGTTGAAGCCAAGCTTAATGAGATCAAGAGGCTG
AGTAGAGTCAGGAGAAGCTTTCATGAGACTATTGACACAAGTCTCTTTGTAATCAGTAGG
TGCACAAACTGCTTCAACTGCCGTGGTGGCTGTTTTAATCTGCACAGGGACGATTTTTTC
TGAATGACTGGTGTTTCTTGATGTTGTGATGCCAACAGCGACAACCATGAGAACGAGCAA
GGCTGTGATAACCCCAGCAATGATGCATTTCTT

Last Updated on 06.12.2018 16:11