Sequences from confirmation amplicon


Confirmation sequences for line SALK_055373c and hit At3g50060 are shown.
Line ID SALK_055373c
Locus At3g50060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18559137
Primers used for PCR 27ea - LBb3, show primer details
   
Sequence Name 93-K111079-SALK_055373C-27ea
GenBank Accession LN486352 [GenBank]
Sequencing primer gene specific primer, 27ea: 5'-TTACTCCACAATCCCTAAAACCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K111079-SALK_055373C-27ea
ATCCCCACTTGTTTTCTGCATTTCCGCCATGTAACTCCTCACTTCAGCTTTTATCATCTC
CTGCACCACCGTCATAAACTCACCTCTACGTCCTTCTCCTCTCTCCTCTACATTAATCTT
CATCTGACTCTCAAATCTCGGAAACATCAACGCGTTGTTGTTATTGTTATGGCTCGAACT
CGTGTTCTCAGCTCCAGGTAGTGACAAACTCAACGAAGTAGGTGGATCCTCCGAAGACGA
AGACATTTCAACCGGTAACGGCTGCGGAACCACCGTAAAACCGCCGGAAATCGGCATTGG
TTTAAACAGCTGAGCAACAGGAGACGACGGTGACGGAATCGTGCTAGAATCACTAACATC
GATTCCGTTAGGACTCTCAGGACTCATGTACAATCCAGTATCCACCGGAGCAAAAGCAGA
ATCAAAGCTAACAGATCTCCTCTTCTTCGGCCGATCCTGATCTTCCTCCGTCTCCGTCAC
CGTCGTAACCGCCACACCTCCGCTGCATTTCCTCTTAAGCGTAGAGTTCCAGTGATTTTT
AACGGCGTTATCCGTACGACCGTTAAGAAGACGAGCAATCGTCGCCCACTTGTTACCAAA
CTGAGCACGGGCGGTTACAATAGTCTCATCTTCCTCCGGCGAGAAAGGACGATGCTCAAC
CTCCGGAGATAACTGATTACACCAACGTAATCTACACGATTTACCAGATCGACCTGGAAT
CGATTTGCTAATCGCAGACCAATTCCTCGGTCCGTATTTCTCAACCATCCTTCGTAGCTG
CTCATCTTCTTCTTGACTCCATGGACCTTTAACACGATCCGCCATTGAAACAGATCTTTT
GTTTGTTTGTTGTAGAGAAGAAGAAGAAGAAGAAGAAGGTGGAGGAAACTGAAGAGAGGA
GAAAAAAGGTATTTATAACAGAGAGAGAGATAGAGAGACCACTTAATAACACATTGCGGA
CGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTG
CCCTTCACCGCCTGGCCCTGA
   
Sequence Name 91-K111078-SALK_055373C-R210
GenBank Accession LN486342 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K111078-SALK_055373C-R210
TACCTTTTTTCTCCTCTCTTCAGTTTCCTCCACCTTCTTCTTCTTCTTCTTCTTCTCTAC
AACAAACAAACAAAAGATCTGTTTCAATGGCGGATCGTGTTAAAGGTCCATGGAGTCAAG
AAGAAGATGAGCAGCTACGAAGGATGGTTGAGAAATACGGACCGAGGAATTGGTCTGCGA
TTAGCAAATCGATTCCAGGTCGATCTGGTAAATCGTGTAGATTACGTTGGTGTAATCAGT
TATCTCCGGAGGTTGAGCATCGTCCTTTCTCGCCGGAGGAAGATGAGACTATTGTAACCG
CCCGTGCTCAGTTTGGTAACAAGTGGGCGACGATTGCTCGTCTTCTTAACGGTCGTACGG
ATAACGCCGTTAAAAATCACTGGAACTCTACGCTTAAGAGGAAATGCAGCGGAGGTGTGG
CGGTTACGACGGTGACGGAGACGGAGGAAGATCAGGATCGGCCGAAGAAGAGGAGATCTG
TTAGCTTTGATTCTGCTTTTGCTCCGGTGGATACTGGATTGTACATGAGTCCTGAGAGTC
CTAACGGAATCGATGTTAGTGATTCTAGCACGATTCCGTCACCGTCGTCTCCTGTTGCTC
AGCTGTTTAAACCAATGCCGATTTCCGGCGGTTTTACGGTGGTTCCGCAGCCGTTACCGG
TTGAAATGTCTTCGTCTTCGGAGGATCCACCTACTTCGTTGAGTTTGTCACTACCTGGAG
CTGAGAACACGAGTTCGAGCCATAACAATAACAACAACGCGTTGATGTTTCCGAGATTTG
AGAGTCAGATGAAGATTAATGTAGAGGAGAGAGGAGAAGGACGTAGAGGTGAGTTTATGA
CGGTGGTGCAGGAGATGATAAAAGCTGAAGTGAGGAGTTACATGGCGGAAATGCAGAAAA
CAAGTGGTGGATTCGTCGTCGGAGGTTTATACGAATCCGGCGGCAATGGTGGTTT

Last Updated on 06.12.2018 16:11