Sequences from confirmation amplicon


Confirmation sequences for line SALK_055597c and hit At2g13820 are shown.
Line ID SALK_055597c
Locus At2g13820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:5775326
Primers used for PCR 57us - LBb3, show primer details
   
Sequence Name 09-K117668-SALK_055597C-R210
GenBank Accession LN484533 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117668-SALK_055597C-R210
AAAATAACATGTTACAAAGGACATTTACGTAATTACACGGGNTTCTCCAACATATTTCCG
ACAAGATACTGTGACTCAAGGTTAGTTTGTACATTCTTTTCAAATCTATAGTCATTTATN
TATAAATACTCTTTTTTAAAAGAAAAAATTGTTTCTATGTATATAAAAAATATAAAAATT
TAATTGAAATTTATCAGTTAAAATTTTATGTTTTTAATAGCTAGAATAAGATTCAAACAT
ATCTTTGAATAGATTGTATAGCCAGATTTTATGCCATTTAACCAAACCTAATTGAAAAAC
CGGAATTCAACAATAGTTATTGATTTAATTACATAGACAGAAAATATAAATATAAATAAT
ACTTACTATTTAATTATAATTTGTTAATCTAAAACCAAACCAAAAAGGATCAAATCAAAA
TTCTCAAACTAAACAATAAAAAATCTCTACTTAATTCATAAATTCACCAAAATCGAAAAA
TCTAGTCTCTTGACTTAAGTATGAAATAACACATACCAGGGGCAGTAGCAGGAGGATCTC
CAGAGACAGAAACTGCAACACAACAATTTCAAAAGCT
   
Sequence Name 46-K117669-SALK_055597C-57us
GenBank Accession LN485421 [GenBank]
Sequencing primer gene specific primer, 57us: 5'-TTGTTGAATCAGCTTTTGAAATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117669-SALK_055597C-57us
CCCCTGGTATGTGTTATTTCATACTTAAGTCAAGAGACTAGATTTTTCGATTTTGGTGAA
TTTATGAATTAAGTAGAGATTTTTTATTGTTTAGTTTGAGAATTTTGATTTGATCCTTTT
TGGTTTGGTTTTAGATTAACAAATTATAATTAAATAGTAAGTATTATTTATATTTATATT
TTCTGTCTATGTAATTAAATCAATAACTATTGTTGAATTCCGGTTTTTCAATTAGGTTTG
GTTAAATGGCATAAAATCTGGCTATACAATCTATTCAAAGATATGTTTGAATCTTATTCT
AGCTATTAAAAACATAAAATTTTAACTGATAAATTTCAATTAAATTTTTATATTTTTTAT
ATACATAGAAACAATTTTTTCTTTTAAAAAAGAGTATTTATAATAAATGACTATAGATTT
GAAAAGAATGTACAAACTAACCTTGAGTCACAGTATCTTGTCGGAAATATGTTGGAGAAC
ACGTGTAATTACGTAAATGTCCTTTGTAACATGTTATTTTTTGGTGTAAACAAATTGACG
CTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11