Sequences from confirmation amplicon


Confirmation sequences for line SALK_055713c and hit At3g16920 are shown.
Line ID SALK_055713c
Locus At3g16920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5777418
Primers used for PCR 87ns - LBb3, show primer details
   
Sequence Name 66-K114703-SALK_055713C-87ns
GenBank Accession LN485906 [GenBank]
Sequencing primer gene specific primer, 87ns: 5'-AAGAAACTTCAAAACCAAGCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K114703-SALK_055713C-87ns
CTCTTCTCTACACAAATTCCGAAACTTAATTCCTCCTAAAACGAACACGACACTACTCTA
TACATATATGTTTGTATATAAAGTTATACATACAATGGTCACATTGATAGACGAAGAAAA
AGACAAAAAAAAATCAACCATGGTTTCAAAACCGCTATTCTCCCTATTGCTTCTCACGGT
TGCATTGGTTGTGTTTCAAACCGGAACCCTTGTCAACGCTAAAAATTCCAAGCCTTCGAG
TTCAACGAGAAAGCCTCTGGTGAAGATTGTGAAAGGTAAAAAATTATGCGACAAAGGATG
GGAATGCAAAGGATGGTCCAAGTATTGTTGCAACCATACAATCTCCGATTTTTTTGAGAC
ATATCAATTCGAGAATCTATTCTCTAAGCGTAACAGTCCAGTGGCTCACGCCGTCGGCTT
TTGGGATTACCGATCATTCATTACTGCGGCTGCCGAGTACCAGCCTCTTGGTTTCGGTAC
TGCTGGTGAGAAACTTCAAGGGATGAAGGAAGTCGCTGCGTTTCTTGGCCACGTTGGGAG
CAAAACCTCAGGTAAGTTTAATTTTATGTAAACAAATTGACGCTAAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCGGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGT
TTGCCCCAGCAGGCGAAAATCCTGTTTAATGGTGGTTC
   
Sequence Name 57-K114702-SALK_055713C-R210
GenBank Accession LN485669 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K114702-SALK_055713C-R210
AATTAAACTTACATGAGGTTTTGCTCCCAACGTGGCCAAGAAACGCAGCGACTTCCTTCA
TCCCTTGAAGTTTCTCACCAGCAGTACCGAAACCAAGAGGCTGGTACTCGGCAGCCGCAG
TAATGAATGATCGGTAATCCCAAAAGCCGACGGCGTGAGCCACTGGACTGTTACGCTTAG
AGAATAGATTCTCGAATTGATATGTCTCAAAAAAATCGGAGATTGTATGGTTGCAACAAT
ACTCGGACCATCCTTTGCATTCCCATCCTTTGTCGCATAATTTTTTACCTTTCACAATCT
TCACCAGAGGCTTTCTCGTTGAACTCGAAGGCTCGGAATCTTCAGCGTTGACAAGGGTTC
CGGTTTGAAACACAACCAATGCAACCGTGAGAAGCAATAGGGAGAATAGCGGTTTCGAAA
CCATGGTTGATTTTTTTTTGTCTTTTTCTTCGTCTATCAATGTGACCATTGTATGTATAA
CTTTATATACAAACATATATGTATAGAGTAGTGTCGTGTTCGTTTTAGGAGGAATTAAGT
TTTCGGAATTTGGTGGTAGGAGAAGAGTTGCTTTTCGTGAGTTTTTTTTTGCTTGGTT

Last Updated on 06.12.2018 16:11