Sequences from confirmation amplicon


Confirmation sequences for line SALK_055721c and hit At1g50960 are shown.
Line ID SALK_055721c
Locus At1g50960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18889566
Primers used for PCR 45wm - LBb3, show primer details
   
Sequence Name 43-K118709-SALK_055721C-45wm
GenBank Accession LN485363 [GenBank]
Sequencing primer gene specific primer, 45wm: 5'-TGAAACCTCTGAAAGAATGATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118709-SALK_055721C-45wm
CGGGAGAGTGGCGCTAGGGTTTCCCCAACGGTAACTATTCTTCGATAAGTCCGAAAAACG
TTCTCTGACTTTCACAGAAAAAGGTTGGTCAAAGAGTTTCTTCTCTTCGAGGAGCATCAT
CTCAAAGACGTCTTTGGGAATTCCATGGTTCACAATTTGAAAAAATCCCCACTCTTTCGC
AGCTGCAACCATTTGTTTCACACATCTTTTGCGTTTGACCTCCTCACCACTAGTTAGATG
GCTGAGATCGATCACAGGAAGCTTTATGCCTGAGGTTTGGATAGTTGATGTGTTTGTATT
GCTCTCACTAGTTGAATTTGGAAATGAGCTTCCGAAAATCGAGCAGAAATTTGTCTTAAA
GGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGAATCGGGCAAAA
   
Sequence Name 18-K118708-SALK_055721C-R210
GenBank Accession LN484798 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118708-SALK_055721C-R210
GTCTTTAGACAATTTCTGCTCGATTTTCGGAAGCTCATTTCCAAATTCAACTAGTGAGAG
CAATACAAACACATCAACTATCCAAACCTCAGGCATAAAGCTTCCTGTGATCGATCTCAG
CCATCTAACTAGTGGTGAGGAGGTCAAACGCAAAAGATGTGTGAAACAAATGGTTGCAGC
TGCGAAAGAGTGGGGATTTTTTCAAATTGTGAACCATGGAATTCCCAAAGACGTCTTTGA
GATGATGCTCCTCGAAGAGAAGAAACTCTTTGACCAACCTTTTTCTGTGAAAGTCAGAGA
ACGTTTTTCGGACTTATCGAAGAATAGTTACCGTTGGGGAAACCCTAGCGCCACTTCTCC
CGCTCAGTACTCCGTTTCGGAAGCGTTTCACATCATTCTTTCAAAGGTTTC

Last Updated on 06.12.2018 16:11