Sequences from confirmation amplicon


Confirmation sequences for line SALK_055969c and hit At5g48680 are shown.
Line ID SALK_055969c
Locus At5g48680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19745030
Primers used for PCR 31ux - LBb3, show primer details
   
Sequence Name 24-K117668-SALK_055969C-R210
GenBank Accession LN484985 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117668-SALK_055969C-R210
CAATTTGTTATAATTTGTGTTATTAAGTTTTCATCTTTTTGCAGGTTGATATGGATGCTC
TTAGGCATATGACAGATGATGACCTCAAAGCTTTGCTCATACCAATGGTAAAGTCCTTGA
TAAGCTTCCACAAGATTTGTTGTTGCAATGCTTGATATCAGCATACCTTACTAATAGTCA
TGGCCTCAGTTTTGCTCCATATTTAATATGGTTGCTATTAAGAAGACAATGTTTAGATTC
GAGTTTCTTTGTTTGAAATGTTGACAAGATGTGTTCTTTTTCAGGGACCTAGGAAGAAGA
TACTTCTTGCTTTGGGAGATAACCGTGGATAGTCCAGCTTCGAGCTTAGGTGCATGATGA
TACACATCTCGGATATTGTCAGAAAGATGCTGCATTTTGACTTTTCATTGTCTTTTTATC
TTTCTCATATGTATCTCAAGAGACAACAAGTAGTTAGAGTTTTGAGATTAAACAGTTGTA
ACCTTTTAACATAGAGAGACAAGAGGAGCAATGTTGGAATGTGTTTAAGATCTTGCTTCC
TTTAGTGAACTTTGTATCAAAGCAATGCGAACAAGAAAACAAACTTGTTGACTGG
   
Sequence Name 61-K117669-SALK_055969C-31ux
GenBank Accession LN485774 [GenBank]
Sequencing primer gene specific primer, 31ux: 5'-TCCAGTCACAAGTTTGTTTTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K117669-SALK_055969C-31ux
AGCAGATCTTAACACATTCCAACATTGCTCCTCTTGTCTCTCTATGTTAAAAGGTTACAA
CTGGTTTAATCTCAAAACTCTAACTACTTGTTGTCTCTTGAGATACATATGAGAAAGATA
AAAAGACAATGAAAAGTCAAAATGCAGCATCTTTCTGACAATATCCGAGATGTGTATCAT
CATGCACCTAAGCTCGAAGCTGGACTATCCACGGTTATCTCCCAAAGCAAGAAGTATCTT
CTTCCTAGGTCCCTGAAAAAGAACACATCTTGTCAACATTTCAAACAAAGAAACTCGAAT
CTAAACATTGTCTTCTTAATAGCAACCATATTAAATATGGAGCAAAACTGAGGCCATGAC
TATTAGTAAGGTATGCTGATATCAAGCATTGCAACAACAAATCTTGTGGAAGCTTATCAA
GGACTTTACCATTGGTATGAGCAAAGCTTTGAGGTCATCATCTGTCATATGCCTAAGAGC
ATCCATATCAACCTGCAAAAAGATGAAAACTTAATAACACAAATTAATAACAAATTTGGG
AATCAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAA
GCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC

Last Updated on 06.12.2018 16:11