Sequences from confirmation amplicon


Confirmation sequences for line SALK_056441c and hit At5g19060 are shown.
Line ID SALK_056441c
Locus At5g19060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6373922
Primers used for PCR 08tw - LBb3, show primer details
   
Sequence Name 17-K116922-SALK_056441C-08tw
GenBank Accession LN484757 [GenBank]
Sequencing primer gene specific primer, 08tw: 5'-ATCTCACCGTTGTTTTACTCTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116922-SALK_056441C-08tw
GCTTGCATTCACAGAACGCTACGAAATTTCAAACTACGGTTTATCAAGAAACCTCACGTT
GTTTTACCGGAGCTCCGGAGATGTGAATTGTCCGATTTCGACAGAGATGTTTGGTCTGGC
CGGTGGATTAGGCTTGGTAAGAACGACGAGTGTGAAATCAGCAATGATGGACGTTACCGT
TGCTTACCAGATGGTTACCGTTGCCGGGAACCATGGTGCGACGGAGCTTTATCGGCGTTA
GAAAGCAATGGTTGGGTTTACTCGAGTCATTGCTCGTTTAAGCTATTCTCTAGTGAATCA
GCTGACAAATAAATTGACGCTTATACAACTTAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GTTTGATGGTGGTTCCGAATGGGGCAAAA
   
Sequence Name 06-K116921-SALK_056441C-R210
GenBank Accession LN484438 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K116921-SALK_056441C-R210
TTCACTAGAGAATAGCTTAAACGAGCAATGACTCGAGTAAACCCAACCATTGCTTTCTAA
CGCCGATAAAGCTCCGTCGCACCATGGTTCCCGGCAACGGTAACCATCTGGTAAGCAACG
GTAACGTCCATCATTGCTGATTTCACACTCGTCGTTCTTACCAAGCCTAATCCACCGGCC
AGACCAAACATCTCTGTCGAAATCGGACAATTCACATCTCCGGAGCTCCGGTAAAACAAC
GTGAGGTTTCTTGATAAACCGTAGTTTGAAATTTCGTAGCGTTCTGTTGAATGCGAAGCG
TGCAGGGCTAAGTTTAAGACCTTGGAAGCGACGGAAGAGTAAAAAGCGGGTGAGAT

Last Updated on 06.12.2018 16:11