Sequences from confirmation amplicon


Confirmation sequences for line SALK_057421c and hit At5g05760 are shown.
Line ID SALK_057421c
Locus At5g05760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1729153
Primers used for PCR 55kk - LBb3, show primer details
   
Sequence Name 71-K113914-SALK_057421C-55kk
GenBank Accession LN486033 [GenBank]
Sequencing primer gene specific primer, 55kk: 5'-ATCCAGGTCAGAGAGAGACTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K113914-SALK_057421C-55kk
TAAACTTTCGAGCATTTTTGTTGAAGCCTCAGTAGTTTGATTTTCTAAGTCCCAAACTCA
TATACGTTGATTACCGTCCTCCTTATGATGTTTAGTATGAAACATATTATGTGTGATTAT
ATCTTAATTAGAATAATAATTACTTTGTTTGCTTTGAATTTTTACTTGAGTTAAATGCTA
TTACATCAGAAGTTTCCTGGTAAAATACAACTGAGTCTTGCAGAAAAATTAAACTTTTTG
ACACTCCACTATCCACACAAAAAAAAGGAGCCCTAGAAGCTTACAAGCTTAACATATGTA
TAGATTTGATACCCCCAGGAACAAGAGATAAAATGGTATTGTTTTAAGCCACAAAGAAGA
GGAAAACAATCAGGAACAAGATGATAACAGCAAAGATCTTCATCATGAGCCATCTATTTG
ACGAAATTCGAGTGAGATGCTGCAGAAGAGCGCTACGTGCTCCCTTAACTAACGATAAAA
TTGTACTGATTGTTTATCAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 60-K113913-SALK_057421C-R210
GenBank Accession LN485745 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113913-SALK_057421C-R210
TATCATCTTGTTCCCGATTCGTTTTCCTCTTCTTCGTGGCTTAAAACAATACCATTTTAT
CTCTTGTTCCTGGGGGTATCAAATCTATACATATGTTAAGCTTGTAAGCTTCTAGGGCTC
CTTTTTTTTGTGTGGATAGTGGAGTGTCAAAAAGTTTAATTTTTCTGCAAGACTCAGTTG
TATTTTACCAGGAAACTTCTGATGTAATAGCATTTAACTCAAGTAAAAATTCAAAGCAAA
CAAAGTAATTATTATTCTAATTAAGATATAATCACACATAATATGTTTCATACTAAACAT
CATAAGGAGGACAGTAATCAACGTATATGAGTTTGGGACTTAGAAATCAAACTACTGAAG
CATCAACAAAAATGCTCAGAAGTTAGTAAGTACCATAGCTATGTCTCAGAACAATCCAAC
CAAGTTTCTTGGATTTGAGTTTACTAACTTGGTGCTTCAGTCT

Last Updated on 06.12.2018 16:11