Sequences from confirmation amplicon


Confirmation sequences for line SALK_057795c and hit At4g09420 are shown.
Line ID SALK_057795c
Locus At4g09420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5962734
Primers used for PCR 12di - LBb3, show primer details
   
Sequence Name 11-K116171w-SALK_057795C-12di
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 12di: 5'-AGATCAATCTGAAAGCAACCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116171w-SALK_057795C-12di
ATTCTTGACACAAAAACGTTGGACTGATCGGGTTGGATCGTCATATGCTAGCACTGAACG
AATTGTTGGATTTAAAATCTAACGAGGAAGTTCGCTTGATTGGGATTTGCGGTCAAGGAG
GCGTCGGCAAGACCACGCTAGCGAGATATGTGTATGAAGAACTATTCAAAAACTTCCATG
CCCATGTGTTTGTAGACAATGCCGGAAAGATTTATAAGCAAGATACCGATGAGTCACATT
CACAAAAATCTTTAACATCAAAGGAGATTCAAGAGGGCACTCAAACGGTGACAAGGACTC
TGACTGTGGCCTCTGATTTTATCAAATCGACGGTTTCACACCAGAGAAGTTTGCTAGTCG
TTGATTGTGTAGACAAATTGCTAGATGACTTGTCGTTTATTAGAAACAAATTGACGCTTA
GACAACTTAATCAAATTGCTAGTAATAACACATAGTTTAACGACGTTTAGACAAAATCAG
CTGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GTTTGATGGTGGTTCCG
   
Sequence Name 05-K116170w-SALK_057795C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116170w-SALK_057795C-R210
CAGCTGATTTTGTCTAACGTCGTTAAACTATGTGTTATTACTAGCAATTTGATTAAGTTG
TCTAAGCGTCAATTTGTTTCTAATAAACGACAAGTCATCTAGCAATTTGTCTACACAATC
AACGACTAGCAAACTTCTCTGGTGTGAAACCGTCGATTTGATAAAATCAGAGGCCACAGT
CAGAGTCCTTGTCACCGTTTGAGTGCCCTCTTGAATCTCCTTTGATGTTAAAGATTTTTG
TGAATGTGACTCATCGGTATCTTGCTTATAAATCTTTCCGGCATTGTCTACAAACACATG
GGCATGGAAGTTTTTGAATAGTTCTTCATACACATATCTCGCTAGCGTGGTCTTGCCGAC
GCCTCCTTGACCGCAAATCCCAATCAAGCGAACTTCCTCGTTAGATTTTAAATCCAACAA
TTCGTTCAGTGCTAGCATATGACGATCCAACCCGATCAGTCCAACGTTTTTTGTTGTCAA
TGAATTGATCTGCTTGTTTGAGACAGTATGCGAGATGGTTGCTTTCAGATTGATCTA

Last Updated on 06.12.2018 16:11