Sequences from confirmation amplicon


Confirmation sequences for line SALK_057940c and hit At2g41310 are shown.
Line ID SALK_057940c
Locus At2g41310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17223284
Primers used for PCR 12vb - LBb3, show primer details
   
Sequence Name 31-K117581-SALK_057940C-12vb
GenBank Accession LN485154 [GenBank]
Sequencing primer gene specific primer, 12vb: 5'-AAACGAAGAAAGCTAATCTAGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117581-SALK_057940C-12vb
ACACTACTGTGTCCGAGACCATCCTGGTTTTGAGCCATTGTTAGTGTGCTATCACCTGAG
TGAAAAGAGAGAACAACCAAGAAACAAGTAAGATAAGAGATGACAAAAGCTATAGTATGA
AACCACATTTCTAGTGTTGCTACTTACCACTATCTGAATGGTTTGAAGCTTCTGATTGAA
TAGGTATTCGATTTAGTGGATCTCCTTGTGTCTGCATTGTCTCTTTTTCCTGATGATAAC
GATGTTGCTGCGGTATTACTCCTAGCAGTCCGTTGTTGTTTGCTTCATATTCAAGAGTTA
GCAGATCTTCTACTGAGAGATTCTGTTGGCATTCTCCATATAGGTCTTGTAGTGGCTTCT
CGGGTTGCGTTAGTCCTCCTTGCAGTCCGTTGTTGTTTGCTTCAGATGCAAGAGTTAGCA
GATCTTCTACTGAGAGATTCTGTTGGCATTCTCCATATAGGTCTTGTAGTGGCTTCTCGG
GTTGCGTTAGTCCTCCTTCAGACCGAGGTTGTGATATCATTGTATTTAGGACGTGATCGA
TCAGTAGAAACCACTTCTTCCATTGCTTTTCTCTTATTACTACTCAACATTGGTTCAAGT
TGTTCGGATTCAATTTCTTGGTGGAGAGGCAAGATTTCAATCACTGATGACTCTTCTTCT
TCTTCTTCTATCTCCGGTTTAGACACCACAATCTCTTCTATGGCAACTGGTTTCTCACTT
TCTTTCTTCAACTTCGTTTTCATCATATGATCAGGATATATTGTGGTGTAAACAAATTGA
CGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGC
   
Sequence Name 15-K117580-SALK_057940C-R210
GenBank Accession LN484706 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117580-SALK_057940C-R210
CGTCATTTGTTACCCACAATATATCCTGATCATATGATGAAAACGAAGTTGAAGAAAGAA
AGTGAGAAACCAGTTGCCATAGAAGAGATTGTGGTGTCTAAACCGGAGATAGAAGAAGAA
GAAGAAGAGTCATCAGTGATTGAAATCTTGCCTCTCCACCAAGAAATTGAATCCGAACAA
CTTGAACCAATGTTGAGTAGTAATAAGAGAAAAGCAATGGAAGAAGTGGTTTCTACTGAT
CGATCACGTCCTAAATACAATGATATCACAACCTCGGTCTGAAGGAGGACTAACGCAACC
CGAGAAGCCACTACAAGACCTATATGGAGAATGCCAACAGAATCTCTCAGTAGAAGATCT
GCTAACTCTTGCATCTGAAGCAAACAACAACGGACTGCAAGGAGGACTAACGCAACCCGA
GAAGCCACTACAAGACCTATATGGAGAATGCCAACAGAATCTCTCAGTAGAAGATCTGCT
AACTCTTGAATATGAAGCAAACAACAACGGACTGCTAGGAGTAATACCGCAGCAACATCG
TTATCATCAGGAAAAAGAGACAATGCAGACACAAGGAGATCCACTAAATCGAATACCTAT
TCAATCAGAAGCTTCAAACCATTCAGATAGTGGTAAGTAGCAACACTAGAAATGTGGTTT
CATACTATAGCTTTTGTCATCTCTTATCTTACTTGTTTCTTGGTTGTTCTCTCTTTTCAC
TCAGGTGATAGCACACTAACAATGGCTCANAACCAGGATGGTCTCGGACACAGTAGTTGT
TTTATTCTCTGTCAATTTACTTCTCTCCCCTAG

Last Updated on 06.12.2018 16:11