Sequences from confirmation amplicon


Confirmation sequences for line SALK_058509 and hit At5g06700 are shown.
Line ID SALK_058509
Locus At5g06700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2063623
Primers used for PCR 19jd - LBb3, show primer details
   
Sequence Name 07-K113376-SALK_058509C-19jd
GenBank Accession LN484463 [GenBank]
Sequencing primer gene specific primer, 19jd: 5'-GTTGTTTGGTTTCTATTCACGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K113376-SALK_058509C-19jd
ATCCGTCGTTGAGATTTGTCTGAAGCAGAGGATGAGATGAAGTGTTGTTCTTTGCATCTG
GGTTGGTTCCGTTTACGATCGGAGACTCAAAGGTTGTGTTTTTAGCAACCGGAGTGTGAG
TTTCCGGCGCCGGTGTTTGTAAAGTTCCGTTTTTGGAATTCGAATTGAGAGGTGGAGAAG
TGGCGTTAACAGAGAGAGAATCTGTGGCGTCACTGGATCTATTGGTGGGTTTGGTTGAAG
TGACATTGGGAAGAATATATGAGAAAATTGAAGAGAACTGAGATCCGGAAGTGTTGTCTG
AAGTTGTGGTTGTGGAGGAGGAAGTGAAGATGTTAGAGAACCAAGGAGAAGATGAGTTTG
GAGAAGGACTAAAAGCAAAGAAGAGAGTGAATGAGACAAAGGTTATGACGAAAGCGTAAG
CGAAAGTTGAGGTTTTTTTGGGTTTTAGAGCGGAGAAGAAGCTCTTGATGTCGGCGGCGG
TTGTTGCTGTTGTTCCTCCGCCGTGGATTGGCATATACTTAACGGCGTCTGACGCCATAG
ACGGAGAGGTGGATGGTCAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 03-K113375-SALK_058509C-R210
GenBank Accession LN484341 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K113375-SALK_058509C-R210
TCCACCTCTCCGTCTATGGCGTCAGACGCCGTTAAGTATATGCCAATCCACGGCGGAGGA
ACAACAGCAACAACCGCCGCCGACATCAAGAGCTTCTTCTCCGCTCTAAAACCCAAAAAA
ACCTCAACTTTCGCTTACGCTTTCGTCATAACCTTTGTCTCATTCACTCTCTTCTTTGCT
TTTAGTCCTTCTCCAAACTCATCTTCTCCTTGGTTCTCTAACATCTTCACTTCCTCCTCC
ACAACCACAACTTCAGACAACACTTCCGGATCTCAGTTCTCTTCAATTTTCTCATATATT
CTTCCCAATGTCACTTCAACCAAACCCACCAATAGATCCAGTGACGCCACAGATTCTCTC
TCTGTTAACGCCACTTCTCCACCTCTCAATTCGAATTCCAAAAACGGAACTTTACAAACA
CCGGCGCCGGAAACTCACACTCCGGTTGCTAAAAACACAACCTTTGAGTCTCCGATCGTA
AACGGAACCAACCCAGATGCAAAGAACAACACTTCATCTCATCCTCTGCTTTCAGACAAA
TCTTCAACGACGGGATCGAATAATCAGTCACGGACCACCGCCGATACTGAAACCGTGAAT
AGA

Last Updated on 06.12.2018 16:11