Sequences from confirmation amplicon


Confirmation sequences for line SALK_058566 and hit At1g55310 are shown.
Line ID SALK_058566
Locus At1g55310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20632428
Primers used for PCR 75hw - LBb3, show primer details
   
Sequence Name 24-K112995-SALK_058566C-75hw
GenBank Accession LN484977 [GenBank]
Sequencing primer gene specific primer, 75hw: 5'-AAACATAAAGTTCTCGGCTTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112995-SALK_058566C-75hw
GGGTCAGACACACCATAATCTAGAGAAGACTAGCAAAAGAGTGACGAATCAATCACTTGC
TTGGTGAACGGTCTTCGTCATACTGGCCCCCATGCGGGCTTTGGCTCCTGCTTCTGGCGG
GTTCAGGAGTGTAGCTTCTTCTGCTTCTCCTCGGACTCCTGCTTCTTCTAGGGCTACGGC
TTATGCTTCTTCTTGGGCTAACGCTCCTATTTCTTTTTGGGCTTGGGCTCCTGCTTCTTC
TTGGGCTACGGCTTATGCTTCTTCTAGGGCTGGGGCTTAAGCTGCGGCTCTTACCTCTGA
CTGGAGTTAAACTGCGACCCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 22-K112994-SALK_058566C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K112994-SALK_058566C-R210
CACGCCAGAACCCGGCAGAGGCAGGAGGCAAAGCCCGCAGGGGGGCGAGGAGGACGAAGA
CAGANCACCAAGACAGGGAG

Last Updated on 06.12.2018 16:11