Sequences from confirmation amplicon


Confirmation sequences for line SALK_059178 and hit At1g13560 are shown.
Line ID SALK_059178
Locus At1g13560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4639428
Primers used for PCR 04vn - LBb3, show primer details
   
Sequence Name 80-K117830-SALK_059178C-04vn
GenBank Accession LN486224 [GenBank]
Sequencing primer gene specific primer, 04vn: 5'-TTTTGCATCAATACAACATTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K117830-SALK_059178C-04vn
GAAAATCACAGCTAACTAAGGCTTTTTTTCTCTTTAGGAGCAAACCTGTGAATATGCAGT
AGCCAAGAAGAACCCATAATTCGTCGACTAGAGGAACCCTGAAATATGAAGTTTCTCAGG
GTGTTAAAGAAAAAAAAGAACCGATGAAGTTTGTGCGGAAAATTTAAAGAAAGAAAACAT
ACCCAGCATTCAATCTTGCGGTTAGCGCATTTGCAAGTGCAAAGGGAAGATAGAGTAGTG
ACTGCATTTTGAGTAAAAAGATTTCACAAGGTTACATTTTCAAAACCAGTATCCAGTAAG
ACGGAGAAATTGGCAAGAAAAAATTATCGGATTTACCATGCACATGTTTGTTTTTAGAAA
TAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 63-K117829-SALK_059178C-R210
GenBank Accession LN485830 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K117829-SALK_059178C-R210
CATTTGTTATTTCTAAAACAAACATGTGCATGGTAAATCCGATAATTTTTTCTTGCCAAT
TTCTCCGTCTTACTGGATACTGGTTTTGAAAATGTAACCTTGTGAAATCTTTTTACTCAA
AATGCAGTCACTACTCTATCTTCCCTTTGCACTTGCAAATGCGCTAACCGCAAGATTGAA
TGCTGGGTATGTTTTCTTTCTTTAAATTTTCCGCACAAACTTCATCGGTTCTTTTTTTTC
TTTAACACCCTGAGAAACTTCATATTTCAGGGTTCCTCTAGTCGACGAATTATGGGTTCT
TCTTGGCTACTGCATATTCACAGGTTTGCTCCTAAAGAGAAAAAAAGCCTTAGTTAGCTG
TTGATTTTTCTTTTTCACTATTCAGTTATTTTGCCCGCTCTAAACCAATGTTGTATTGAT
GCAAA

Last Updated on 06.12.2018 16:11