Sequences from confirmation amplicon


Confirmation sequences for line SALK_059416c and hit At1g27400 are shown.
Line ID SALK_059416c
Locus At1g27400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9515350
Primers used for PCR 16vo - LBb3, show primer details
   
Sequence Name 84-K118049-SALK_059416C-16vo
GenBank Accession LN486277 [GenBank]
Sequencing primer gene specific primer, 16vo: 5'-TGACAATACATTCACGAAATCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K118049-SALK_059416C-16vo
CAGGTCCGATCATATTAATCGAAATGAAGCAATTTCACAAATTCTGAGGAGAGGGGAGGA
CAAGTAGAAGAAAGAAACCTCGGAATCAAGAGGATCGTTCTTGTACTGAGCCTCAGAATT
TTTGCGAGCATGTTCGAACATGATAAGCCTTTCAAAGTCGGCGGTAGAGAACTCCATCTT
CAGATTCAGGTGAAAGAAAATCTAGGGTTCGGTGTTTTTGTTACTGAGAAAAGTCTAAAG
AGTTGTCTCGATGTGAAACAAGTTCCGAGGGTTTTAGCCTACAAGTCGGGTGTTACCAGC
ACATAATTGGGCTTTTTAAGCCTACTTATCAAGATAAGTGGCCCAGAAGGCCCATATGAA
CAAATAGTACTTTAATACACTAGTTCAACTAATGTATTCGCCGATTAGGGTTTCTTCTGT
TTCACTATATATTAAGTGTCTCCAACTACTGAGATCTCTATCCTCTCCGTCCTCTGTTCT
TCTCATTGGCCGCCATGGTAAATGCGTTACACTAGACTCGTCGTTACAACGTTTTCACTT
CTAACTTATGCTCCTATACTTATGTTCTTCGTCTTCTTATTGTTTTTCTTCAGGTGAAGT
ACTCTCAAGAGCCCGATAATACATTGCGGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTC
   
Sequence Name 78-K118048-SALK_059416C-R210
GenBank Accession LN486199 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K118048-SALK_059416C-R210
CGGGCTCTTGAAGTACTTTCACCTGAAGAAAAACAATAAGAAGACGAAGAACATAAGTAT
AGGAGCATAAGTTAGAAGTGAAAACGTTGTAACGACGAGTCTAGTGTAACGCATTTACCA
TGGCGGCCAATGAGAAGAACAGAGGACGGAGAGGATAGAGATCTCAGTAGTTGGAGACAC
TTAATATATAGTGAAACAGAAGAAACCCTAATCGGCGAATACATTAGTTGAACTAGTGTA
TTAAAGTACTATTTGTTCATATGGGCCTTCTGGGCCACTTATCTTGATAAGTAGGCTTAA
AAAGCCCAATTATGTGCTGGTAACACCCGACTTGTAGGCTAAAACCCTCGGAACTTGTTT
CACATCGAGACAACTCTTTAGACTTTTCTCAGTAACAAAAACACCGAACCCTAGATTTTC
TTTCACCTGAATCTGAAGATGGAGTTCTCTACCGCCGACTTTGAAAGGCTTATCATGTTC
GAACATGCTCGCAAAAATTCTGAGGCTCAGTACAAGAACGATCCTCTTGATTCCGAGGTT
TCTTTCTTCTACTTGTCCTCCCCTCTCCTCAGAATTTGTGAAATTGCTTCATTTCGATTA
ATATGATCGGACCTGATATGTAACGAATATTAAGGATGTTTTGTGGAGATGGATTT

Last Updated on 06.12.2018 16:11