Sequences from confirmation amplicon


Confirmation sequences for line SALK_059682 and hit At4g29100 are shown.
Line ID SALK_059682
Locus At4g29100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14344017
Primers used for PCR 46wm - LBb3, show primer details
   
Sequence Name 45-K118709-SALK_059682C-46wm
GenBank Accession LN485404 [GenBank]
Sequencing primer gene specific primer, 46wm: 5'-CTTTCTTAGGCTCTTCACTTGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118709-SALK_059682C-46wm
CTTCTCTTATGCACTGATCGTTCACGAGCTAAAACCATATCAAAAAAAAAAAAAACCACC
GGGATTTTTGGCTTTGGTTAACAAAAAAGGTAACCCCTTGTTTTCTTAACCCCTTTTTAA
AGGGGGGAAAAAAAAAAAAATCTTTGATTTTCCCCTGGGGAAAAGCATTTTTAAACCCCC
CCCAAAGGATTTTGATTTTCCTTTAAAAAAAAAAAACAAGTAGGGGAAAAAATTTGATTT
TTAAAAAATTTTGGGGGTTTTTGGGGTTTTAAAAAATTTTTTGGGTTTTAAAAAAGGGAG
GGGGGGGGGAAAAAAAAGGGGGCCCCCCCCTTTTTTAATTTTAACCCCCTTTTTTTTTAA
AAAAAACGGTAAAAAAAAAAAAAAACAATTTGGCCCTTAAACCATTTAAAAACCCTTTGG
GGACTTTTTTAAGGGCGGGGGGGGGTTTTTTTTTTCCCCGGGGAAAGGGGAAAACGTTTT
TTGCCCTTCCCCCCCGGGCCCTAAAAAATTTCCAAAAAGGGGCCCCCCTTGTTTTCCCCC
CACGGGGAAAAACCCGTTTTGGGGGGGGTTCCAAACCGGGAAAAAAA
   
Sequence Name 20-K118708-SALK_059682C-R210
GenBank Accession LN484869 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118708-SALK_059682C-R210
CATTTGTTTTTTTCTTTTCTACTGTTTTCTTTAAGTAAAGTTGGATTAATATTAAAAGAT
GCAGCGGCCANGCTTTTTTTTCACCCTCCCTTACATTATTATAAACCAAAAAAGTTCGTT
AAAACCAACAAAGCCACTAAATTTATTTAAATATCAACTTTTTCTCCTTACTTGTTTTCT
TCATCTAAGTGAAATTCAAATTCCTTTGGCGGGGTTTTATATATGCTTTTCACTAGGGGA
AATTCAATGATTTTTTTTCTTATCACCTCTTTATATAGGTGTTAGAGAACATGTGTTTAC
TTTTTTTGTTAATCAATGCTATATATCATGATTGTTTTTTTTTTTTTTAAAAGGGTTTTA
CCCCGGAAAAAAACCGGGGAAAAAAAAAAAAAGAATTTTCTCCTTTTTTAGGGAAAAAAA
AAAGGGAAGGGAAAACCCCAAAAAAG

Last Updated on 06.12.2018 16:11