Sequences from confirmation amplicon


Confirmation sequences for line SALK_059686c and hit At1g66180 are shown.
Line ID SALK_059686c
Locus At1g66180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24647497
Primers used for PCR 22dh - LBb3, show primer details
   
Sequence Name 22-K109819-SALK_059686C-22dh
GenBank Accession LN484910 [GenBank]
Sequencing primer gene specific primer, 22dh: 5'-CGGAAATGTTAAGCTTCTTCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K109819-SALK_059686C-22dh
TGTAGCAGAGGATCAAGATTCGGCATTCGTTGACTTTCAGGAAAAGTCAACAAAGAAACG
TATTTGAAACCGTGTGAATTCGGGTTATCACCAAGGTAAAACGAACCGGTTGGAGTAAAA
CCGGGCCGGTTTGATTTGGGAGGGATGCAATAAGAGAACTTCGAAATCTTAGCTTGAGAA
ACAAAAGAGAGACGACCACGGTTCATTCCCAAAATGCCCCTATCATCAGAAGACTCAGTA
GCACAACCAAGAATCAAAGGAGGGGTAATTTCGGTATTTGAGAAAGTGATTTTTTCTTTA
ACGAGATTACCCTCAGCGAAGGTTCCATCAGCGTAGAAGTAAGAGTAGTGACATAACCTG
TTGGAGTCACAAGAAGTAGGAAGGGTAAAATCGGGAATTCTCGGTTTACAAAGAGGATGT
GAGCAAGGCAAAGTTGAGAAAGAAGAAGAAAGAGATGGATNCAATGATGTTTTTGGTTTT
GGAGGAAGCTTTTTACGATGACATTGGATCCAAGAAAGTTGGCTTCCAAATTGACGCTTA
GACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACC
AGTGAGACGGGCAACAGCTG
   
Sequence Name 06-K109818-SALK_059686C-R210
GenBank Accession LN484425 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K109818-SALK_059686C-R210
ATTTGGAGCCAACTTTCTTGGATCCATGTCATCGTAAAAAGCTTCCTCCAAACCAAAAAC
ATCATTTGATCCATCTCTTTCTTCTTCTTTCTCAACTTTGCCTTGCTCACATCCTCTTTG
TAAACCGAGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACTCCAACAGGTTATGTCA
CTACTCTTACTTCTACGCTGATGGAACCTTCGCTGAGGGTAATCTCGTTAAAGAAAAAAT
CACTTTCTCAAATACCGAAATTACCCCTCCTTTGATTCTTGGTTGTGCTACTGAGTCTTC
TGATGATAGGGGCATTTTGGGAATGAACCGTGGTCGTCTCTCTTTTGTTTCTCAAGCTAA
GATTTCGAAGTTCTCTTATTGCATCCCTCCCAAATCAAACCGGCCCGGTTTTACTCCAAC
CGGTTCGTTTTACCTTGGTGATAACCCGAATTCACACGGTTTCAAATACGTTTCTTTGTT
GACTTTTCCTGAAAGTCAACGAATGCCGAATCTTGATCCTCTTGCTTACACTGTTCCTAT
GATTGGGATTAGATTTGGTTTGAAGAAGCTAAACATTTCCG

Last Updated on 06.12.2018 16:11