Sequences from confirmation amplicon


Confirmation sequences for line SALK_059944c and hit At1g75060 are shown.
Line ID SALK_059944c
Locus At1g75060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28182396
Primers used for PCR 39um - LBb3, show primer details
   
Sequence Name 09-K117319-SALK_059944C-39um
GenBank Accession LN484532 [GenBank]
Sequencing primer gene specific primer, 39um: 5'-CACCAAGTAGAAGAAATTTGAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117319-SALK_059944C-39um
CGAGATTTTGAGTGTGAGTCACAAGAAACTTCCCTGTACAAGCCTTTTGAGATAACCGCG
ATGATCTGTGTCCTCTCTTCTTCGACTTATGAGGCTTCAAAGTGTCCTCAGTTGCTGCAA
AAGACAATCAAAGAACGGTCTTTATGAAACATTTCAAAAAGACTAAAATTTGGTACTTTA
TTGCAGAGACAAGGAACCAAACTTACTCATATCGGAGGGATGATTCCACTGTGTAGAATG
GTCGACTTCAAGATCATTGTCTTCTTCGTTCCCTGTAGGATGTTCAAGAACACTAAGCGC
ATTCCTCTGCAACTTCACTTCAATTCCATTAGTCAAAACCTGAAAATTTAAAGCAAAAAG
GACAAAGATTAAAAATTGAACTCCACAAACAAAGTAAAAAAAATGACGAAAAAAAAAATA
TACTTTGAATTTGAGAAAACAAATAACACATTAAACAAATATACTTTAAATTGAGTTTGT
TACAAATTTGTTATCCAAAATATAAATATAAAGTAAAAATATACTTTAAATTGAGTTTGT
TACAAATTTGTTATCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 25-K117318-SALK_059944C-R210
GenBank Accession LN485013 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117318-SALK_059944C-R210
ACCACAATATATCCTGGATACAAATTTGTAACAAACTCAATTTAATGTATATTTTTACTT
TATATTTATATTTTGGATAACAAATTTGTAACAAACTCAATTTAATGTATATTTGTTTAA
TGTGTTATTTGTTTTCTCAAATTCAATGTATATTTTTTTTTTCGTCATTTTTTTTACTTT
GTTTGTGGAGTTCAATTTTTAATCTTTGTCCTTTTTGCTTTAAATTTTCAGGTTTTGACT
AATGGAATTGAAGTGAATTTGCAAAGGAATGCCCTTAGTGTTCTTGAACATCCTACAGGG
AACAAAAAAAACAATGATCTTGAAGTCAACCATTCTACACAGTGAAATCATCCCTCCAAT
ATGAGTAAGTTTGGTTCCTTGTCTCTGCAATAAAGTACCAAATTTTAGTCTTTTTGAAAT
GTTTCATAAAAACCGTTCTTTGATTGTCTTTTGCAGCAACTGAGGACACTTTGAACCCTC
ATAAGTCGAAAAAAAGAGGACACAGATCATCGCGGTTATCTCAAAAGGCTTTGTACAGGG
AAGTTTCTTGTGACTCACACTCAAAAATCTCAAGTATTACTCCTCGTCTGAACATGGTAA
GGCCTCAAATTTCTTCTACTTGGTG

Last Updated on 06.12.2018 16:11