Sequences from confirmation amplicon


Confirmation sequences for line SALK_060037c and hit At1g72210 are shown.
Line ID SALK_060037c
Locus At1g72210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27181553
Primers used for PCR 40um - LBb3, show primer details
   
Sequence Name 10-K117319-SALK_060037C-40um
GenBank Accession LN484565 [GenBank]
Sequencing primer gene specific primer, 40um: 5'-TTCGAGGAAGAAGCTTATGAATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117319-SALK_060037C-40um
TTGTGACCACCAAACATGCCCAGTACACATATATATATACACACATTCACACATGTGTAG
GCATATGTCATCTTTTTGGTCCCTGATTCTTACAAGAATCAATATTATGATAGTACTCAA
AATTTTGTCTTTTTTAATAAATAAGAAATGATGATTTTGACCCCCCAATAATTGAGGCCA
GCCCTAACCAAGTAAAAAAGAACGAGAAAAAAGGAACGATTTTTATTTTTGTTCTTGGAA
AACCTTTTTTGGCCCAGACAAATAATTCACAACATGTACTAATAAGAGACACATTAAATG
CACAAAACATTTGTGATTGGAAAAACAATCAATGAATAATCATTGAACCAAAACCAACAA
TGGGAGTAATGATTAGTCACCCATTAATTATTAGATACCAATTTAATTTGAATTTGCAAA
TTCAACCCACTAACCCTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 26-K117318-SALK_060037C-R210
GenBank Accession LN485043 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117318-SALK_060037C-R210
GTCATTTGTTAGGGTAGTGGGTTGATTTGCAATTCAAATTAAATTGGTATCTAATAATTA
ATGGGTGACTAATCATTACTCCCATTGTTGGTTTTGGTTCAATGATTATTCATTGATTGT
TTTTCCAATCACAAATGTTTTGTGCATTTAATGTGTCTCTTATTAGTACATGTTGTGAAT
TATTTGTCTGGGCCAAAAAAGGTTTTCCAAGAACAAAAATAAAAATCGTTCCTTTTTTCT
CGTTCTTTTTTACTTGGTTAGGGCTGGCCTCAATTATTGGGGGGTCAAAATCATCATTTC
TTATTTATTAAAAAAGACAAAATTTTGAGTACTATCATAATATTGATTCTTGTAAGAATC
AGGGACCAAAAAGATGACATATGCCTACACATGTGTGAATGTGTGTATATATATATGTGT
ACTGGGCATGTTTGGTGGTCACAACACTGTGGATGCACTGTGCACGGCATAACGAAATGA
CGCCACTTGTTTCACGACACATTCATAAGCTTCTTC

Last Updated on 06.12.2018 16:11