Sequences from confirmation amplicon


Confirmation sequences for line SALK_061534c and hit At2g45920 are shown.
Line ID SALK_061534c
Locus At2g45920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18900429
Primers used for PCR 62fr - LBb3, show primer details
   
Sequence Name 25-K112129-SALK_061534C-62fr
GenBank Accession LN485003 [GenBank]
Sequencing primer gene specific primer, 62fr: 5'-GGTCTACCCCTAGTCCCTGTAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K112129-SALK_061534C-62fr
TTTTTTAAATGATATTTGTGTTTTGTCACACTCTTGTGAGTTTTAACAGGAACTGAGATT
GTGAGAACAATCACATGTCACAAGCTCTAATATATCTGGTGATGGTATAATGCAGGGAAT
CTATCATGGGCAACACCTCTCTAGAGTATGCATCTACAAGTTCTGGTCAAGATTCAGTCC
GTTCACGAGGATCTGTTATACCTTCGCGCCAATTCACGATATCTAGAGGAAATGGAAATG
TGTATCAGTTAGCTGTCTTTGAAGCTGAGAAATCGAAAAAAGAAGCAAGCTTGGAGGCTT
TCAAGCATCAAGAAGTTGTAAAAGAGAAAAATGAAGCAATCAAAAGGGTAACATCTTCTT
GGAATCTCACATAAGCTATTGCCATTGCCAAAGAGAAAACAAATTGACGCTNAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 09-K112128-SALK_061534C-R210
GenBank Accession LN484520 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K112128-SALK_061534C-R210
CATTTGTTTTCTCTTTGGCATGGCAATAGCTTATGTGAGATTCCAGAAGATGTTACCCTT
TTGATTGCTTCATTTTTCTCTTTTACAACTTCTTGATGCTTGAAAGCCTCCAAGCTTGCT
TCTTTTTTCGATTTCTCAGCTTCAAAGACAGCTAACTGATACACATTTCCATTTCCTCTA
GATATCGTGAATTGGCGCGAAGGTATAACAGATCCTCGTGAACGGACTGAATCTTGACCA
GAACTTGTAGATGCATACTCTAGAGAGGTGTTGCCCATGATAGATTCCCTGCATTATACC
ATCACCAGATATATTAGAGCTTGTGACATGTGATTGTTCTCACAATCTCAGTTCCTGTTA
AAACTCACAAGAGTGTGACAAAACACAAATATCATTTTAAAAAAAACTTCTGAAGTAGTA
TTTACAGTTGTACAGAGATTACAGGGAC

Last Updated on 06.12.2018 16:11