Sequences from confirmation amplicon


Confirmation sequences for line SALK_062374c and hit At3g15720 are shown.
Line ID SALK_062374c
Locus At3g15720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5325792
Primers used for PCR CG69 - R204, show primer details
   
Sequence Name 59-K106282-SALK_062374C-R210
GenBank Accession LN485705 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K106282-SALK_062374C-R210
GTCATTTGATCAAGTAATTTGAGATACGTACCTTACGATCTTTGGCATTATCGGAATCGC
CACCGTTATAGAACTGATCGATTATGATTGGATTTTCAACATTGTCTAGAGTAATTCCAT
TGAAAGTAATCATTCTCGCGTAACCCGATCCGCCCTATGTATATATAACCAGAACTTATT
TAAAAGAACAAGAAAAAGAAACACATGTAAGGTGACTGAAACATGAGAGTTTAATAAGAA
AATTCAAAAGTACCTGCCAAGTTTTGATCCGAGCTCCATTCATAGTTCCTCTAAAGTTAC
AGTTTTGGACACATACATTCTCCACTGTAGCTGTCTCTCCATCTTTTCCCAAGCTTCCTA
TGCTATACATTTTACAATATACATGGTTTATCTATATATATGTAAAACATAGATAACATT
TTGTAGTACATTTTTAATTTGGTTAACCTTATCCCATGGCCTGGTCCGCAATCTATACCG
GAAATGTGANGGTTAGA
   
Sequence Name 75-K106283-SALK_062374C-CG69
GenBank Accession LN486122 [GenBank]
Sequencing primer gene specific primer, CG69: 5'-GTCTAACATCCACATTTCCGGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K106283-SALK_062374C-CG69
TTACCAATTAAAATGTACTACAAAATGTTATCTATGTTTTACATATATATAGATAAACCA
TGTATATTGTAAAATGTATAGCATAGGAAGCTTGGGAAAAGATGGAGAGACAGCTACAGT
GGAGAATGTATGTGTCCAAAACTGTAACTTTAGAGGAACTATGAATGGAGCTCGGATCAA
AACTTGGCAGGTACTTTTGAATTTTCTTATTAAACTCTCATGTTTCAGTCACCTTACATG
TGTTTCTTTTTCTTGTTCTTTTAAATAAGTTCTGGTTATATATACATAGGGCGGATCGGG
TTACGCGAGAATGATTACTTTCAATGGAATTACTCTAGACAATGTTGAAAATCCAATCAT
AATCGATCAGTTCTATAACGGTGGCGATTCCGATAATGCCAAAGATCGTAAGGTACGTAT
CTCAAATTTACTTTGATCAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAACGGNTCCACG
Primers used for PCR 59xs - LBb3, show primer details
   
Sequence Name 86-K119375-SALK_062374C-59xs
GenBank Accession LN486302 [GenBank]
Sequencing primer gene specific primer, 59xs: 5'-CAATGCAGATGTAACGATCATACC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K119375-SALK_062374C-59xs
ACCATTATATCATATTAAGCTTCTCAAGATGTGACATTTGGTGAGATTTAGATACCAATT
TCTCGAAATCTAAAACGCAAATGAGAGTAAAATCAATTGATATCGAAACGAACCGAGAGT
GATAGCCTGAGTGAGAACTTGACGGATCTTGATAGATTTGATGGAATCTACAGTTTCTCC
GATGAAACCCATGATTGATTCTTCCTTCGATTTCGTGTTTTTCCCAGCGAAAGTGATAGG
ATCAGATCCCTTTGACCCTTCTCCTTCAAACGCGTTTCAGTGGAGAAGAGAATGAAACAC
AGATTTGATTTGATTCACGAGCGTTTCTGCAAATCGGTTTAAATATCGAAATGTCGGTTT
ACTACTGTTAGTTAAACCGGTTTAAATATTAATGAATATGTTTGAATTTAAACCTTTGAC
TATATCTTTATTACGGAAATACCACCATTTATGGCTATAAATAGCAATAGATTTGATTTT
GATATATGTGAAATTTTGGGGTTTTGTTATAAATGGTAAGAAAATCAAGTAAATGAAAAA
ATAATCTCATTGAAAAAAATAGGCTGTTGAGAATAAAAACAAGGGTTTCATTTTTTTTCT
TTGATAATTGGTTAATTAAACCATTCAGAAAATAGTTCTCCTGTTTGTAACATAGCGGAG
TTTGAATCCATGTGGATGTGGTGACACTTATAAAAACAATTAGATTACAAATTTTACTCG
CATAATATATGTAACCTATCTAGCTAAAATATATGTAACCAAAGAGATAAATGAAACTAA
TATGGCAATATTGTAAACTCTAAGTTGTTTCCCTCTGCTTTGAAAACCCATATGGGATCT
TGCATCGGTTGTGTTGTTGTTTCT
   
Sequence Name 82-K119374-SALK_062374C-R210
GenBank Accession LN486252 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K119374-SALK_062374C-R210
CAAAATTTAAGTTTTTTTGTTCGGTTTTGTAGTCGTCGGCAGTGGAAGTGAGCAAAGTGG
TGTTTAGTAATTTCATTGGGACGTCAAAGTCAGAGTACGGTGTTGACTTCCGATGCAGCG
AGAGAGTTCCATGCACAGAGATTTTCTTGAGAGACATGAAAATAGAAACGGCATCATCAG
GATCAGGACAAGTCGCACAAGGACAGTGTTTAAACGTGAGAGGTGCGTCTACAATTGCTG
TACCAGGTCTAGAATGTTTAGAACTTTCCACAGATATGTTTTCATCGGCGCAATTGCTGG
AACAAACTTGCATGTCGGCACAATCAGTGCAACCAAGAACAACAACACAACCGATGCAAG
ATCCAATATGGGTTTTTCAAAGCAGAGGGAAACAACTTAGAGTTTACAATATTGCCATAT
TAGTTTCATTTATCTCTTTGGTTACATATATTTTAGCTAGATAGGTTACATATATTATGC
GAGTAAAATTTGTAATCTAATTGTTTTTATAAGTGTCACCACATCCACATGGATTCAAAC
TCCGCTATGTTACAAACAGGAGAACTATTTTCTGAATGGTTTAATTAACCAATTATCAAA
GAAAAAAAATGAAACCCTTGTTTTTATTCTCAACAGCCTATTTTTTTCAATGAGATTATT
TTTTCATTTACTTGATTTTCTTACCATTTATAACAAAACCCCAAAATTTCACATATATCA
AAATCAAATCTATTGCTATTTATAGCCATAAATGGTGGTATTTCCGTAATAAAGATATAG
TCAAAGGTTTAAATTCAAACATATTCATTAATATTTAAACCGGTTTAACTAACAGTAGTA
AACCGACATTTCGATATTTAAACCGATTTGCAGAAACGCTCGTGAAATCAATCAAATCTG
TGTTTCATTCTCTT

Last Updated on 06.12.2018 16:11