Sequences from confirmation amplicon


Confirmation sequences for line SALK_062789 and hit At1g15700 are shown.
Line ID SALK_062789
Locus At1g15700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5402936
Primers used for PCR 28ea - LBb3, show primer details
   
Sequence Name 66-K110715-SALK_062789C-28ea
GenBank Accession LN485894 [GenBank]
Sequencing primer gene specific primer, 28ea: 5'-AGACATTGATTTTCCTCTGAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K110715-SALK_062789C-28ea
GCTTTGGTGTTGTTCTGGCGATAAGGATTGTGTGGTGGTTTCACAATGCAGTGACCAAGA
AAGCTACTCTGCGAGTTCAAGAGTTGAAGCAGAGAGGAATAGACTGTGTTGTGATAAGTG
TAGGTAAGAAAGGGAATGCTTATTTTAGTCGGAGAGATGAATTTGATGTTGATAAATGTA
TTGAAGGAGGAGGTGTTTTCCCAACGACTAAAGAAGCTCAAGTTATTGCAGATGATGTGT
TTTCCTTGTTTGTCAGTGAAGAGGTTGATAAAGTTGAGCTTGTTTACACAAAGTTTGTGT
CTTTGGTGAAATCTGATCCTGTGATCCACACTCTGTTGCCTTTGTCGATGAAAGGAGAGT
CTTGTGATGTGAAAGGTGAGTGTGTTGATGCTATCGAGGATGAGATGTTTAGGCTAACGA
GCAAAGATGGGAAGTTAGCTGAAAACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 50-K110714-SALK_062789C-R210
GenBank Accession LN485505 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K110714-SALK_062789C-R210
GTCATTTGTTTTCGCTAACTTCCCATCTTTGCTCGTTAGCCTAAACATCTCATCCTCGAT
AGCATCAACACACTCACCTTTCACATCACAAGACTCTCCTTTCATCGACAAAGGCAACAG
AGTGTGGATCACAGGATCAGATTTCACCAAAGACACAAACTTTGTGTAAACAAGCTCAAC
TTTATCAACCTCTTCACTGACAAACAAGGAAAACACATCATCTGCAATAACTTGAGCTTC
TTTAGTCGTTGGGAAAACACCTCCTCCTTCAATACATTTATCAACATCAAATTCATCTCT
CCGACTAAAATAAGCATTCCCTTTCTTACCTACACTTATCACAACACAGTCTATTCCTCT
CTGCTTCAACTCTTGAACTCGCAGAGTAGCTTTCTTGGTCACTGCATTGTTGAAACCACC
ACACAATCCTTTATCGCCAGTAACAACAACCAAAGCAACTCTCTTCACGGGTCTAACAAT
GCTCAGAGGAAAATCAATGTC

Last Updated on 06.12.2018 16:11