Sequences from confirmation amplicon


Confirmation sequences for line SALK_063265c and hit At4g16600 are shown.
Line ID SALK_063265c
Locus At4g16600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9351231
Primers used for PCR 20ht - LBb3, show primer details
   
Sequence Name 77-K112669-SALK_063265C-20ht
GenBank Accession LN486172 [GenBank]
Sequencing primer gene specific primer, 20ht: 5'-CAGTAATAAGATGCGTCCATTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K112669-SALK_063265C-20ht
CTTAGCGCGGTGCGGTTATCGGGAGGAGGACGAAAGAGAGGCTGATTGAGTAAATCAGAG
AAGTAGCTGACAAGAAAGCCTTGATCAGCTCCATCAGGGTTATCTCTCTTTACTTCAAGC
TCATGAAGCATGTCTCTAAAGACCTCCATTGATGGCTGCAACACAAAGAGACCAGTGTGG
AAGATGCAAGGGTTGATGAAGACAGCACAAAATTGGCCACACTGGAAGAGCTCGTCGGTG
TTCTTGAGAAAGAGATTGTCGACATCAAGCATTACAACACGGTCATAATCAGAGAGAGAC
CAAGCGTAGAGCTTGTTTAGACTAAGCTTGAATCTGTTGTCGAAGTTGGTTTGTTTCTTG
TATGGATTCTCAAGATTCTCTACTCTCACTACTTTAGCTCCATCTTCTTCTTCCCTACAT
ACCTCATTAATGAATTATAATAAGATCAAAACAGATTTTTTTTGCATTATGTATAAAATG
CGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 67-K112668-SALK_063265C-R210
GenBank Accession LN485931 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K112668-SALK_063265C-R210
TTTATCATATGCAAAAAATCTGTTTTGATCTTATTATAATTCATTAATGAGGTATGTAGG
GAAGAAGAAGATGGAGCTAAAGTAGTGAGAGTAGAGAATCTTGAGAATCCATACAAGAAA
CAAACCAACTTCGACAACAGATTCAAGCTTAGTCTAAACAAGCTCTACGCTTGGTCTCTC
TCTGATTATGACCGTGTTGTAATGCTTGATGTCGACAATCTCTTTCTCAAGAACACCGAC
GAGCTCTTCCAGTGTGGCCAATTTTGTGCTGTCTTCATCAACCCTTGCATCTTCCACACT
GGTCTCTTTGTGTTGCAGCCATCAATGGAGGTCTTTAGAGACATGCTTCATGAGCTTGAA
GTAAAGAGAGATAACCCTGATGGAGCTGATCAAGGCTTTCTTGTCAGCTACTTCTCTGAT
TTACTCAATCAGCCTCTCTTTCGTCCTCCTCCCGATAACCGCACCGCGCTTAAGGGACAT
TTTAGGCTTCCTTTGGGATATCAAATGGACGCATCTTATTACT

Last Updated on 06.12.2018 16:11