Sequences from confirmation amplicon


Confirmation sequences for line SALK_063269c and hit At3g57540 are shown.
Line ID SALK_063269c
Locus At3g57540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21301629
Primers used for PCR 11ii - LBb3, show primer details
   
Sequence Name 30-K112623-SALK_063269C-11ii
GenBank Accession LN485129 [GenBank]
Sequencing primer gene specific primer, 11ii: 5'-GTTTAAGAGACAAGACGCCGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K112623-SALK_063269C-11ii
AGCTACTCTTGGATGAAGAAATCGAGGTGACTCATTTTTTTCTATTTTACGAATATCTTA
GTTAGATCCGATCGATTAAGAGAACAAGATGACTTATAACATGCTTTTCTTGAAACTGTT
TGCTTTTACATGTATAACAAAATAATTTAGAAAAACTATGTAACGTTTTCAAATTGCAAA
CGATAAACGTGGACAAGCGTTTTTTACAAATTATAAAATGCATATTTTATCCAACCAAAA
AAAAAAAATCAAAATGTAAAAATTAGGGGTTTATTTAAAAAAAAACCAAAACCCCAAATA
ACCAATAAATCCTTGAAGGGGGGTAATATATCAAATATTTAAAAGGGGAAAAAGCTTTCC
AAAAAACGGACAAAATAAACTTCAATTTTGTCTTTGAAAAAAAAAATAAGGCAATGGATA
CATATTTTTGAATGCAAAGAAAACTGGAAAATAGGAAACCAAAGGCAATGGAAAAAACAC
AAAACAAAGGGGCAAAACCTCAAAGAAAGGCGAAGAAAAGAAAACCCACGGCGAAGGGTA
AACAAGGAACGGAGGTTGCAAGGTTTCTTAAATTTGCTAATCTCAGGAAACCCTTTGAAC
AACCTCCGGCCAAACAATAATTCTTCTCTTTCCATTTAAAACTATAAGGATATATGGGGG
TGTAAACAAATTGACCCTAAAACAACTAAATAACACATTGCGAACTTTTTTAAGGTACGG
GGGGGGTTTTTCTTTTCACCATTGAAACGGGCAACAGCTAATTGCCTTTCACCGCCGGGC
CCTA
   
Sequence Name 06-K112622-SALK_063269C-R210
GenBank Accession LN484432 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K112622-SALK_063269C-R210
TTTGTTTACCACAATATATCCTGATAGTTAAACTGAAGAGAGAAGAATGATCGTTTGGCC
GGAGGTCGTCCAACGGCTCTCATGAGATTAGCAACTTCAAGAACCCTCGCAACCTCCGTC
CCTCGTTTACCTTCCGCCGTGGCTCTCCTCTCCTCCGCCTTCCTCTGAGCTTTTGCCACT
TTGTTTTGTGTTTTCTCCATCGCCTTCGCTCTCCTATCTTCCAGTTTCCTCTGCATCCAC
ATATATGTATCAATCGCATAATCTATTATTCCAAAGACAACATCGACGTCTATTTAGTCA
GTCTTTTGGATAGCTTTATCACATTTCAAATATTTGATATATCACCACCATTCAAGGATC
TATCGTTTATTTGTGTTTCTGCTTTTTTTTCAAATAAACCCATAATTCTTACATTTTGAT
TTTTTTTTTTGGGTGGGAAAAAATAGGCTTTTTAAAATTGGAAAAAAACCCTGGCCCACT
TTTATCGTTGGCAATTTAAAAACTTTACAAAGTTTTTCAAAATTATTTTGTTATACATGT
AAAACCAAACGTTTTCAAAAAAAGCAGGTTAAAAGTCATCTGGTTCTCTAAACCGATGGG
ATCTAACTAAAATTTTCGAAAAAAAAAAAAAAAGGAGTCCCCTCGATTTTCTTCACCCAA
AATTTAGCTCTTTAAACCGGCTCTTTCAACCAACCGTTAATAACGGCTTCTTG

Last Updated on 06.12.2018 16:11