Sequences from confirmation amplicon


Confirmation sequences for line SALK_063337 and hit At3g56710 are shown.
Line ID SALK_063337
Locus At3g56710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21007056
Primers used for PCR 57qz - LBb3, show primer details
   
Sequence Name 14-K115992-SALK_063337C-57qz
GenBank Accession LN484675 [GenBank]
Sequencing primer gene specific primer, 57qz: 5'-AAACCCATCAAAGTTCGTTACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115992-SALK_063337C-57qz
CTTCTAGTTCAGAGAGCTCGTTCAAGAACTCACAGGCCAAGACGCCGTCGATCTTCAACC
GGAGCCCATCTATTCCCCTTCCTCCGACGACCACAACCTTTCTCCACCGGCGGAGAATCT
TGCGCCACGTGTTCTTCATCAGGAGCCGTTCGGTGAGAGAGACAGTGATTGTTATGAGCC
GTTGAATGCGGAAGACATGTTTTTACCTGATCAGATGTCGGCAGGTTTTTCTGGGTTTTT
CTCTAATGGGTTTTACAATGTCAATGACTTTGGAAGCATCGATTCTATGATATATTGTGG
TGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTG
GGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC
CCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTT
TGATGGTGGTTCCG
   
Sequence Name 05-K115991-SALK_063337C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115991-SALK_063337C-R210
TTGCGGGTGTTTTATCTAGAGTCCTCTGGTTCTCTGCTGCGTGTGTCCAAAACCCCGTGT
GTGGGTGGTGCTGAAAAAAA

Last Updated on 06.12.2018 16:11