Sequences from confirmation amplicon


Confirmation sequences for line SALK_063470c and hit At2g35930 are shown.
Line ID SALK_063470c
Locus At2g35930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15083559
Primers used for PCR 18gg - LBb3, show primer details
   
Sequence Name 04-K112347-SALK_063470C-R210
GenBank Accession LN484368 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K112347-SALK_063470C-R210
TTTGTTTCCCACANTTATCCTGCCCATGGGGAAGGAACGACACAAGGCCGTGGAAGCTGG
AGTAATCTCTGTGATCATCGAGCTTCTCATGGACGAGAGCTTCACATCAGAGAGGAGAGG
TCCAGAGATGGCGATGGTGGTTCTTGATCTGTTGTGTCAGTGTGCGGAGGGACGAGCCGA
GTTCTTGAACCACGGAGCAGCCATAGCGGTGGTGTGCAAGAAGATACTTAGGGTTTCACA
GACAGCAAGCGATAGAGCGGTTAGGGTTTTGTTGTCGGTGGGAAGGTTCTGCGCAACGCC
TGCTTTGTTGCACGAGATGTTACAGTTGGGGGTTGTAGCGAAGCTTTGTCTTGTGCTTCA
AGTAAGCTGTGGAGGTAAGACCAAAGAGAAGGCAAAGGAGTTGCTTAAGTTGCACGCTAG
GGTCTGGAAGGACTCGCCTTGTCTCCCAAAAAACATGATTCTTGCATATCCCTGCTGATG
AAAACGAAGAAAACAATACAAACAAGTCCAATTCTCAGTGAGAGCAATCACATATATGAG
AGATGCTGTTGAAAAAACTAAAGGAGTATTCAATTATTCATATCATTAATCCAATTTTCC
TTAGGTTTCTTATTTCGTACTTTTGATGTTCTCAATGTAGATAATAGTGGAAAATGTTCA
TCAAATATTTTTTTTTATGAAAAACTGCAAAGATATCATATATAAAATCACAAGTTACCA
GGCTATTGTCACTTTCAT
   
Sequence Name 13-K112348-SALK_063470C-18gg
GenBank Accession LN484640 [GenBank]
Sequencing primer gene specific primer, 18gg: 5'-AAATGAAAGTGACAATAGCCTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K112348-SALK_063470C-18gg
TCTTTGCGTTTTTCTAAAAAAAATATTTGATGAACATTTTCCACTATTATCTACATTGAG
AACATCAAAAGTACGAAATAAGAAACCTAAGGAAAATTGGATTAATGATATGAATAATTG
AATACTCCTTTAGTTTTTTCAACAGCATCTCTCATATATGTGATTGCTCTCACTGAGAAT
TGGACTTGTTTGTATTGTTTTCTTCGTTTTCATCAGCAGGGATATGCAAGAATCATGTTT
TTTGGGAGACAAGGCGAGTCCTTCCAGACCCTAGCGTGCAACTTAAGCAACTCCTTTGCC
TTCTCTTTGGTCTTACCTCCACAGCTTACTTGAAGCACAAGACAAAGCTTCGCTACAACC
CCCAACTGTAACATCTCGTGCAACAAAGCAGGCGTTGCGCAGAACCTTCCCACCGACAAC
AAAACCCTAACCGCTCTATCGCTTGCTGTCTGTGAAACCCTAAGTATCTTCTTGCACACC
ACCGCTATGGCTGCTCCGTGGTTCAAGAACTCGGCTCGTCCCTCCGCACACTGACACAAC
AGATCAAGAACCACCATCGCCATCTCTGGACCTCTCCTCTCTGATGTGAAGCTCTCGTCC
ATGAGAAGCTCGATGATCACAGAGATTACTCCAGCTTCCACGGCCTTGTGTCTGTTCCTT
CCCCATGGGCAGGATATATTGTGGTGTAAACAAATTGACGCTNAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCNGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT
GCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA

Last Updated on 06.12.2018 16:11