Sequences from confirmation amplicon


Confirmation sequences for line SALK_063674 and hit At1g15870 are shown.
Line ID SALK_063674
Locus At1g15870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5457149
Primers used for PCR 17rb - LBb3, show primer details
   
Sequence Name 74-K116055-SALK_063674C-17rb
GenBank Accession LN486113 [GenBank]
Sequencing primer gene specific primer, 17rb: 5'-CATCAATTTGCGTATGAGATGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K116055-SALK_063674C-17rb
GATGTCGACTTTTTGTGTATCAGATTACCAAATGCTTAACAATTACAAATTGAAAGTTTC
AGAATACCTGAAGAATAGGCGAGTGATCAAGCTCGGACTGAATCTCCAAACGAATTAATC
TGAGAATGTTACCCTCGAAAGCTGTTCTCCGCATTTCTGAAACGTAACTCCCCGAGCTGA
GAAACTTGCGGCTGCTTAAGAAAATATAGCTTTGATTTGACTGAACACATATTCTAGGGT
TTCTGTACGCAATCGAAGTCGATGAACTAAATATGAAACTTCTTCTCAGACATCTGAGGA
GGCGAGCCATTGACCAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTCCGAATGGGGGCAAAAT
   
Sequence Name 47-K116054-SALK_063674C-R210
GenBank Accession LN485439 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K116054-SALK_063674C-R210
CTCTGCAGGGCCAGGCGGTGAAGGGCTATCAGATGTTGCCCGTCTCACTGGAGAAAAGAA
AAACCACCCCGATACATTAAAAACGTCCGCAATGTGTTGTTACGTTGTCTAAGCGTCAAT
TTGGTCAATGGCTCGCCTCCCCAGATGTCTGAAAAGAAGTTTCATATTTAGTTCATCGAC
TTCTATTGCGTACAGAAACCCTAGAATATGTGTTCGGTCACATCCGAGCTCTATTTTCTT
AAGCAGCCGCTTGTTTCTCAACTCGGGGAGTTACGTTTCATAAATGCGGATAACAGCTAT
CCAGGGTAACATTCTCAGATGAATTCGTTTGGAGATTCGGGCCGAGCTTGATCTCTCCCC
TATACATCGGGTATTCTGAAACTTTCATTTTGTAATTGTTAAGCATTTGGTAATCTGATA
CTACCAAAACTCGACTTCTTTAGAAACTGGAATCTAGGATTGAAT

Last Updated on 06.12.2018 16:11