Sequences from confirmation amplicon


Confirmation sequences for line SALK_063974c and hit At1g20780 are shown.
Line ID SALK_063974c
Locus At1g20780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7220288
Primers used for PCR 49cy - LBb3, show primer details
   
Sequence Name 43-K109566-SALK_063974C-49cy
GenBank Accession LN485349 [GenBank]
Sequencing primer gene specific primer, 49cy: 5'-TTATTTAAAGGGTGCGAAGAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K109566-SALK_063974C-49cy
GTTTGTGTGAGCTTAAACGTTTTACAAATTGGTTTAATCTATGAACTCAATATATATTTG
ATGCAAAAGTAAGCACAACTCCCTCCGCAACACACATAAGCCTTTTTAATCTGAGGCCAA
TCAAATGATTTCGATTTCCAACGTAGGATTTATAAACAAATAAGAATCGGAATTTACAAT
ATATACCCAGAAAAAAACTGGATGCCTATTAACAAGTAATCCCATCAAAACAAAAACCAC
AGTTTTAAAATCACTATAACTCACTGGACAGTTTCAAAGAGATTTTCCCTATGCGATGTT
TGGGAATATACTTGAGAAGTTTGGGATCTTATCAATGTGTTTCAACGCGTTCTCAGCGAT
CTGTCTCGTTCTAAAATCAGCGTTTTGGAAAGCGTCCACAAGTGCAGCGCTCAGGCTCTG
TTCCTCTGCCACCTCTCTTGCTATGTCTTCAATCCGCAAGATCCTTTCCACCATCCAAAC
CGCTCTCCTTGTCAGCCTCTCTGTTCGGTTCTCCCTCAGAACGTTTAGTATATGCCGTAT
CCCATCCGCTTCATCGAGTATCTTGACCCCTTTCTCTACATCTAACCCGTCTTCTAGTAA
GCTCGATAGACAAATTGACAACTTAGACAACACATTGACGCTTAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAACCTGGTTT
GCCCCA
   
Sequence Name 34-K109565-SALK_063974C-R210
GenBank Accession LN485199 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K109565-SALK_063974C-R210
GTCATGTGTTGTCTAGTTGTCAATTTGTCTATCGAGCTTACTAGAAGACGGGTCAGTTGT
AGAGAAAGGGGTCAAGATACTCGATGAAGCGGATGGGATACGGCATATACTAAACGTTCT
GAGGGAGAACCGAACAGAGAGGCTGACAAGGAGAGCGGTTTGGATGGTGGAAAGGATCTT
GCGGATTGAAGACATAGCAAGAGAGGTGGCAGAGGAACAGAGCCTGAGCGCTGCACTTGT
GGACGCTTTCCAAAACGCTGATTTTAGAACGAGACAGATCGCTGAGAACGCGTTGAAACA
CATTGATAAGATCCCAAACTTCTCAAGTATATTCCCAAACATCGCATAGGGAAAATCTCT
TTGAAACTGTCCAGTGAGTTATAGTGATTTTAAAACTGTGGTTTTTGTTTTGATGGGATT
ACTTGTTAATAGGCATCCAGTTTTTTTCTGGGTATATATTGTAAATTCCGATTCTTATTT
GTTTATAAATCCTACGTTGGAAATCGAAATCATTTGATTGGCCTCAGATTAAAAAGGCTT
ATGTGTGTTGCGGAGGGAGTTGTGCTTACTTTTGCATCAAATATATATTGAGTTCATAGA
TTAAACCAATTTGTAAAACGTTTTAAGCTTCACACAAACTGATTTGAGATTTGGGAAGAA
CAAAAACAACTCTTTCTTCGCACCCTTTAAAT

Last Updated on 06.12.2018 16:11